概述

  • 产品名称Anti-PKC epsilon抗体
    参阅全部 PKC epsilon 一抗
  • 描述
    兔多克隆抗体to PKC epsilon
  • 特异性ab63638 detects endogenous levels of total PKC epsilon protein.
  • 经测试应用适用于: IHC-Fr, IHC-P, ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic non phosphopeptide derived from human PKC epsilon around the phosphorylation site of serine 729 (G-F-SP-Y-F).

  • 阳性对照
    • HeLa, Jurkat and NIH 3T3 cell extracts, all treated with PMA (125ng/ml, 30mins). FFPE human normal pancreas tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab63638 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 25165884
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 结构域The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • 翻译后修饰Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KPCE_HUMAN antibody
    • MGC125656 antibody
    • MGC125657 antibody
    • nPKC epsilon antibody
    • nPKC-epsilon antibody
    • PKCE antibody
    • Pkcea antibody
    • PRKCE antibody
    • Protein kinase C epsilon antibody
    • Protein kinase C epsilon type antibody
    see all

Anti-PKC epsilon antibody 图像

  • ICC/IF image of ab63638 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63638, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PKC epsilon antibody (ab63638) at 1/500 dilution + Human Cell Lysate- Whole cell (HMEC) at 20 µg

    Secondary
    Donkey anti rabbit Conjugation: HRP at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa


    Exposure time : 10 minutes

    This image is courtesy of an anonymous abreview.

  • Anti-PKC epsilon antibody (ab63638) at 1/500 dilution + Mouse Cell lysate - whole cell (NIH3T3) at 20 µg

    Secondary
    Donkey anti rabbit HRP linked Conjugation: HRP at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa


    Exposure time : 10 minutes

    This image is courtesy of an anonymous abreview.

  • IHC image of PKC epsilon staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63638, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PKC epsilon antibody (ab63638)参考文献

This product has been referenced in:
  • Foerster F  et al. Targeting the actin cytoskeleton: selective antitumor action via trapping PKC?. Cell Death Dis 5:e1398 (2014). IHC-P, IHC-Fr . Read more (PubMed: 25165884) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH3T3)
Loading amount 20 µg
Specification NIH3T3
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HMEC)
Loading amount 20 µg
Specification HMEC
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"