Anti-PKC epsilon抗体[17] (ab11724)

概述

  • 产品名称Anti-PKC epsilon抗体[17]
    参阅全部 PKC epsilon 一抗
  • 描述
    小鼠单克隆抗体[17] to PKC epsilon
  • 特异性ab11724 recognises the epsilon isoform of protein kinase C. This antibody recognises the sequence NQEEFKGFSYFGEDLMP at the C terminus of PKC epsilon.
  • 经测试应用适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    C-terminal peptide of human PKC epsilon.

  • 常规说明This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.


    This is a calcium-independent, phospholipid-dependent enzyme specific for serine and threonine. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumour promoters.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tissue Culture Supernatant
  • 纯度Tissue culture supernatant
  • Primary antibody说明This is a calcium-independent, phospholipid-dependent enzyme specific for serine and threonine. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumour promoters.
  • 克隆单克隆
  • 克隆编号17
  • 骨髓瘤Sp2/0
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/10.
WB 1/10 - 1/500. Predicted molecular weight: 88.4 kDa.

靶标

  • 功能This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 结构域The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • 翻译后修饰Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KPCE_HUMAN antibody
    • MGC125656 antibody
    • MGC125657 antibody
    • nPKC epsilon antibody
    • nPKC-epsilon antibody
    • PKCE antibody
    • Pkcea antibody
    • PRKCE antibody
    • Protein kinase C epsilon antibody
    • Protein kinase C epsilon type antibody
    see all

Anti-PKC epsilon antibody [17] (ab11724)参考文献

ab11724 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I am sorry but we do not recommend a positive control lysate for this antibody. However, following some literature searching I have found a publication where a purified form of the protein was used to demonstrate express...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"