Anti-PKC epsilon抗体[17] (ab11724)

概述

  • 产品名称
    Anti-PKC epsilon抗体[17]
    参阅全部 PKC epsilon 一抗
  • 描述
    小鼠单克隆抗体[17] to PKC epsilon
  • 特异性
    ab11724 recognises the epsilon isoform of protein kinase C. This antibody recognises the sequence NQEEFKGFSYFGEDLMP at the C terminus of PKC epsilon.
  • 经测试应用
    适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    C-terminal peptide of human PKC epsilon.

  • 常规说明
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.


    This is a calcium-independent, phospholipid-dependent enzyme specific for serine and threonine. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumour promoters.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: Tissue Culture Supernatant
  • 纯度
    Tissue culture supernatant
  • Primary antibody说明
    This is a calcium-independent, phospholipid-dependent enzyme specific for serine and threonine. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumour promoters.
  • 克隆
    单克隆
  • 克隆编号
    17
  • 骨髓瘤
    Sp2/0
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/10.
WB 1/10 - 1/500. Predicted molecular weight: 88.4 kDa.

靶标

  • 功能
    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 结构域
    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • 翻译后修饰
    Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KPCE_HUMAN antibody
    • MGC125656 antibody
    • MGC125657 antibody
    • nPKC epsilon antibody
    • nPKC-epsilon antibody
    • PKCE antibody
    • Pkcea antibody
    • PRKCE antibody
    • Protein kinase C epsilon antibody
    • Protein kinase C epsilon type antibody
    see all

文献

ab11724 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry. I am sorry but we do not recommend a positive control lysate for this antibody. However, following some literature searching I have found a publication where a purified form of the protein was used to demonstrate express...

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Thank you for your e-mail regarding the antibody ab11724. I appreciate you taking the time to express your disappointment with this product, your opinion is very valuable to us and we take your comments very seriously. Thank you for the images which al...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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