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A synthetic phospho-peptide from residues surrounding Tyrosine 311 of human PKC delta.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab76181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500 - 1/5000. Predicted molecular weight: 77 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Dot blot analysis of PKC delta (pY311) phospho peptide (Lane 1) and PKC delta non-phospho peptide (Lane 2) using ab76181 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: 3 minutes.
ab76181 at 1/20 immunoprecipitating PKC delta (phospho Y311) in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
Lane 1 (input): HeLa treated with 10mM H2O2 for 1 hour whole cell lysate (10µg)
Lane 2 (+): ab76181 + HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76181 in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
For western blotting, ab76181 was used as a dilution of 1/200 followed by ab131366 VeriBlot for IP (HRP) at a dilution of 1/1000.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This image is courtesy of an abreview submitted by Bryan Niedenberger Tomar Ghansah
Blocking- 5% Milk for 1 hour at 25°C.
A non-specific band was observed at 80 kDa.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"