Anti-PKC delta (phospho T505)抗体(ab60992)

概述

  • 产品名称
    Anti-PKC delta (phospho T505)抗体
    参阅全部 PKC delta 一抗
  • 描述
    兔多克隆抗体to PKC delta (phospho T505)
  • 特异性
    Detects endogenous levels of PKC delta only when phosphorylated at threonine 505.
  • 经测试应用
    适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Human PKC delta (internal sequence).

性能

应用

Our Abpromise guarantee covers the use of ab60992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).

靶标

  • 功能
    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 结构域
    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
    The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
  • 翻译后修饰
    Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
  • 细胞定位
    Cytoplasm. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CVID9 antibody
    • D14Ertd420e antibody
    • Kinase PKC delta antibody
    • KPCD antibody
    • KPCD_HUMAN antibody
    • MAY 1 antibody
    • MAY1 antibody
    • MGC49908 antibody
    • nPKC delta antibody
    • nPKC-delta antibody
    • PCKd antibody
    • PKC d antibody
    • PKC delta antibody
    • PKCD antibody
    • PKCdelta antibody
    • PRKC D antibody
    • PRKC delta antibody
    • Prkcd antibody
    • Protein Kinase C delta antibody
    • Protein kinase C delta type antibody
    • Protein kinase C delta VIII antibody
    • Protein Kinase Cdelta antibody
    • Tyrosine protein kinase PRKCD antibody
    see all

图片

  • All lanes : Anti-PKC delta (phospho T505) antibody (ab60992) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3cells, treated with UV (15mins).
    Lane 2 : Extracts from NIH/3T3cells, treated with UV (15mins) with immunizing phosphopeptide.


    Predicted band size : 77 kDa
    Observed band size : 77 kDa
  • ab60992 staining human normal tonsil tissue. Staining is localised to cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

文献

ab60992 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cervical Adenocarcinoma, Epithelial)
Loading amount
40 µg
Specification
HeLa Cervical Adenocarcinoma, Epithelial
Treatment
100nM Insulin for 1hr
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Tomar Ghansah

Verified customer

提交于 Feb 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
Loading amount
40 µg
Specification
C57BL/6 Mouse splenocytes
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Tomar Ghansah

Verified customer

提交于 Oct 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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