概述

  • 产品名称
    Anti-PKC delta (phospho S645)抗体
    参阅全部 PKC delta 一抗
  • 描述
    兔多克隆抗体to PKC delta (phospho S645)
  • 特异性
    This antibody detects PKC delta only when phosphorylated at serine 645.
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human PKC delta around the phosphorylation site of serine 645 (R-L-SP-Y-S).

  • 阳性对照
    • IHC-P: Human breast carcinoma tissue. WB: MCF7 cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab47773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

靶标

  • 功能
    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 结构域
    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
    The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
  • 翻译后修饰
    Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
  • 细胞定位
    Cytoplasm. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CVID9 antibody
    • D14Ertd420e antibody
    • Kinase PKC delta antibody
    • KPCD antibody
    • KPCD_HUMAN antibody
    • MAY 1 antibody
    • MAY1 antibody
    • MGC49908 antibody
    • nPKC delta antibody
    • nPKC-delta antibody
    • PCKd antibody
    • PKC d antibody
    • PKC delta antibody
    • PKCD antibody
    • PKCdelta antibody
    • PRKC D antibody
    • PRKC delta antibody
    • Prkcd antibody
    • Protein Kinase C delta antibody
    • Protein kinase C delta type antibody
    • Protein kinase C delta VIII antibody
    • Protein Kinase Cdelta antibody
    • Tyrosine protein kinase PRKCD antibody
    see all

图片

  • This image shows human breast carcinoma stained with ab47773 at 1/50 dilution. Right hand image shows tissue treated with the immunogenic phospopeptide, whereas the left hand image is untreated.
  • All lanes : Anti-PKC delta (phospho S645) antibody (ab47773) at 1/500 dilution

    Lane 1 : MCF7 cell extract, untreated
    Lane 2 : MCF7 cell extract treated with immunogenic phosphopeptide


    Predicted band size : 77 kDa

文献

This product has been referenced in:
  • Misuth M  et al. The flashlights on a distinct role of protein kinase C d: Phosphorylation of regulatory and catalytic domain upon oxidative stress in glioma cells. Cell Signal 34:11-22 (2017). WB ; Human . Read more (PubMed: 28237688) »

See 1 Publication for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab47773.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册