Anti-PKC Consensus抗体(ab40652)

概述

  • 产品名称Anti-PKC Consensus抗体
  • 描述
    兔多克隆抗体to PKC Consensus
  • 经测试应用适用于: ELISAmore details
  • 种属反应性
    与反应: Rat, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to Bovine thyroglobulin, corresponding to amino acids 543-550 of PKC Consensus

性能

  • 形式Lyophilised:Reconstitute with 0.1 ml of 10 mg/ml BSA in PBS.
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • Concentration information loading...
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.

    With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Serine/threonine protein kinases phosphorylate the OH group of serine or threonine (which have similar sidechains). Protein kinase C is actually a family of protein kinases consisting of ~10 isozymes. They are divided into three subfamilies: conventional (or classical), novel, and atypical based on their second messenger requirements. While serine/threonine kinases all phosphorylate serine or threonine residues in their substrates, they select specific residues to phosphorylate on the basis of residues that flank the phosphoacceptor site, which together comprise the consensus sequence. Since the consensus sequence residues of the substrate to be phosphorylated make contact with the catalytic cleft of the kinase at several key amino acids (usually through hydrophobic forces and ionic bonds), a kinase is usually not specific to a single substrate, but instead can phosphorylate a whole "substrate family" having common recognition sequences. The consensus sequence of protein kinase C enzymes is similar to that of protein kinase A, since it contains basic amino acids close to the Ser/Thr to be phosphorylated. Their substrates are MARCKS proteins, MAP kinase, transcription factor inhibitor IkappaB, the vitamin D3 receptor VDR, Raf kinase, calpain, and the epidermal growth factor receptor.
    • 细胞定位Cytoplasmic and Plasma membrane
    • 数据库链接
    • 别名
      • PKC A antibody
      • PKCA antibody
      • PRKCA antibody

    Anti-PKC Consensus antibody (ab40652)参考文献

    ab40652 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab40652.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"