RabMAb

Anti-PKC beta 2抗体[Y125] (ab32026)

概述

  • 产品名称Anti-PKC beta 2抗体[Y125]
    参阅全部 PKC beta 2 一抗
  • 描述
    兔单克隆抗体[Y125] to PKC beta 2
  • 特异性ab32026 recognises PKC beta 2, it does not cross react with human PKC beta 1. Immunogen's homology with PKC alpha is 77.8%. Cross-reactivity with PKC alpha has not been tested.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Cow, Pig不与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to residues near C terminus of human PKC beta 2

  • 表位ab32026 reacts with an epitope located in the region near the C terminus of PKC beta 2.
  • 阳性对照
    • K562 cell lysate, HeLa cell lysate and human breast carcinoma
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-PKC beta 2 antibody (Alexa Fluor® 488) [Y125] (ab195251)

    Anti-PKC beta 2 antibody (Alexa Fluor® 647) [Y125] (ab195252)

    Anti-PKC beta 2 antibody (HRP) [Y125] (ab194906)

    Anti-PKC beta 2 antibody (Phycoerythrin) [Y125] (ab210976)

     

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号Y125
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab32026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/60.

靶标

  • 功能Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
  • 细胞定位Cytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KPCB_HUMAN antibody
    • PKC Beta antibody
    • PKC-B antibody
    • PKC-beta antibody
    • PKCB antibody
    • Prkcb antibody
    • PRKCB II antibody
    • PRKCB2 antibody
    • Protein kinase C beta antibody
    • Protein kinase C beta type antibody
    see all

Anti-PKC beta 2 antibody [Y125] 图像

  • All lanes : Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution

    Lane 1 : K562 whole cell lysates at 20 µg
    Lane 2 : Active human PKC beta 2 full length protein at 0.02 µg
    Lane 3 : Human fetal brain at 20 µg
    Lane 4 : NIH/3T3 whole cell lysates at 20 µg
    Lane 5 : Raw264.7 whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa


    Exposure time : 15 seconds

    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

    The PKC beta 2 recombinant protein is GST-tagged, so the molecular weight is higher.

  • Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + Anti-ICAM1 antibody [15.2] (ab20) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa


    Exposure time : 3 minutes

    Blocking Buffer: 5% NFDM/TBST

    Diluting Buffer: 5% NFDM/TBST

  • Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + K562 cell lysate

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
  • Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + Recombinant human PKC beta 2 protein (ab60841) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 77 kDa
    Observed band size : 103 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 seconds

    Blocking Buffer: 5% NFDM/TBST

    Diluting Buffer: 5% NFDM/TBST

  • All lanes : Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/1000 dilution

    Lane 1 : MCF-7 whole cell lysates
    Lane 2 : HeLa whole cell lysates
    Lane 3 : K562 whole cell lysates
    Lane 4 : Recombinant human PKC alpha protein (ab55672)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa


    Exposure time : 30 seconds

    Blocking Buffer: 5% NFDM/TBST

    Diluting Buffer: 5% NFDM/TBST

  • ab32026, at a 1/100 dilution, staining human PKC beta 2 in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue
  • ab32026, at a 1/250 dilution, staining human PKC beta 2 in HeLa cells by Immunoflourescence
  • Overlay histogram showing HeLa cells stained with ab32026 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32026, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-PKC beta 2 antibody [Y125] (ab32026)参考文献

This product has been referenced in:
  • Qu C  et al. Trimeric G protein-CARMA1 axis links smoothened, the hedgehog receptor transducer, to NF-?B activation in diffuse large B-cell lymphoma. Blood 121:4718-28 (2013). Read more (PubMed: 23632891) »
  • Chao C  et al. Epstein-Barr virus infection and expression of B-cell oncogenic markers in HIV-related diffuse large B-cell Lymphoma. Clin Cancer Res 18:4702-12 (2012). IHC-P ; Human . Read more (PubMed: 22711707) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Cow Cell lysate - other (Cerebral microvascular endothelial cells)
Gel Running Conditions Reduced Denaturing (8% Bis-Tris)
Loading amount 10 µg
Specification Cerebral microvascular endothelial cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 14 2015

Thank you for contacting us.

This antibody has been tested in IP so it will more likely to work in Co-IP also. However we cannot confirm it because we do not have tested data. The experimental conditions have to be determined by the end user...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7)
Loading amount 100000 cells
Specification MCF7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 May 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (GLC16 Cells)
Loading amount 40000 cells
Specification GLC16 Cells
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"