Anti-PKC beta 1 + PKC beta 2抗体(ab71123)

概述

  • 产品名称Anti-PKC beta 1 + PKC beta 2抗体
    参阅全部 PKC beta 1 + PKC beta 2 一抗
  • 描述
    兔多克隆抗体to PKC beta 1 + PKC beta 2
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human PKC beta 1 + PKC beta 2 aa 311-341 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P05771

  • 阳性对照
    • Human placenta lysate; Paraffin embedded human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种���IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).

靶标

  • 功能Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
  • 细胞定位Cytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KPCB_HUMAN antibody
    • PKC beta antibody
    • PKC-B antibody
    • PKC-beta antibody
    • PKCB antibody
    • Prkcb antibody
    • PRKCB I antibody
    • PRKCB II antibody
    • PRKCB1 antibody
    • PRKCB2 antibody
    • Protein kinase C beta 1 antibody
    • Protein kinase C beta 2 antibody
    • Protein kinase C beta antibody
    • Protein kinase C beta type antibody
    • protein kinase C, beta 1 polypeptide antibody
    see all

Anti-PKC beta 1 + PKC beta 2 antibody 图像

  • Anti-PKC beta 1 + PKC beta 2 antibody (ab71123) at 1/100 dilution + human placenta lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 150 kDa,20 kDa,30 kDa,40 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-PKC beta 1 + PKC beta 2 antibody (ab71123) at 1/100 dilution

    Lane 1 : Non-transfected HEK293 cell lysate
    Lane 2 : HEK293 cell lysate transiently transfected with PKC beta 2

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 77 kDa
  • ab71123 at 1/50 dilution, staining PKC beta 1 + PKC beta 2 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining

Anti-PKC beta 1 + PKC beta 2 antibody (ab71123)参考文献

ab71123 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab71123.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"