RabMAb

Anti-PKC alpha (phospho T638)抗体[E195] (ab32502)

概述

  • 产品名称Anti-PKC alpha (phospho T638)抗体[E195]
    参阅全部 PKC alpha 一抗
  • 描述
    兔单克隆抗体[E195] to PKC alpha (phospho T638)
  • 特异性The antibody only detects PKC alpha phosphorylated on Threonine 638.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.

  • 阳性对照
    • 293 cell lysate and normal brain tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab32502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/20000. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa).
IHC-P 1/250.
ICC/IF 1/500.
IP 1/40.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    Anti-PKC alpha (phospho T638) antibody [E195] 图像

    • All lanes : Anti-PKC alpha (phospho T638) antibody [E195] (ab32502) at 1/20000 dilution

      Lane 1 : 293 cell lysate, untreated
      Lane 2 : 293 cell lysate, treated with TPA.


      Predicted band size : 77 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of untreated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution.
    • Immunohistochemical staining of Phosphatase-treated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution

    Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)参考文献

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    We have not yet tested this item for cross reactivity against other PKC family members. BLASTs and immunogen alignment that I have performed show that this product, has 85% and 66% homology against mouse PKC beta and gamma respectively. Alignment again...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"