概述

  • 产品名称
    Anti-PKA R2抗体[Y116]
    参阅全部 PKA R2 一抗
  • 描述
    兔单克隆抗体[Y116] to PKA R2
  • 特异性
    The antibody recognises PKA RII. It does not cross-react with other cAMP-dependent kinase.
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the C-terminus of human cAMP-dependent protein kinase type II-alpha regulatory subunit.

  • 阳性对照
    • K562 cell lysate, HeLa cells and normal breast tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
IP 1/50.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
    • 组织特异性
      Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
    • 序列相似性
      Belongs to the cAMP-dependent kinase regulatory chain family.
      Contains 2 cyclic nucleotide-binding domains.
    • 翻译后修饰
      Phosphorylated by the activated catalytic chain.
    • 细胞定位
      Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • cAMP dependent protein kinase regulatory subunit alpha 2 antibody
      • cAMP dependent protein kinase regulatory subunit RII alpha antibody
      • cAMP dependent protein kinase type II alpha regulatory chain antibody
      • cAMP dependent protein kinase type II alpha regulatory subunit antibody
      • cAMP-dependent protein kinase type II-alpha regulatory subunit antibody
      • KAP2 antibody
      • KAP2_HUMAN antibody
      • MGC3606 antibody
      • PKR 2 antibody
      • PKR2 antibody
      • PRKA R2 antibody
      • PRKAR 2 antibody
      • PRKAR2 antibody
      • PRKAR2A antibody
      • Protein kinase A RII alpha subunit antibody
      • Protein kinase cAMP dependent regulatory type II alpha antibody
      see all

    图片

    • Anti-PKA R2 antibody [Y116] (ab32514) at 1/50000 dilution + K562 cell lysate

      Predicted band size : 45 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human normal breast tissue using ab32514 at 1/100 dilution.
    • Immunofluorescent staining of HeLa cells using ab32514 at 1/100 dilution.

    文献

    This product has been referenced in:
    • Hillman P  et al. Dissociation between AKAP3 and PKARII promotes AKAP3 degradation in sperm capacitation. PLoS One 8:e68873 (2013). WB ; Cow . Read more (PubMed: 23894359) »
    • Gu L  et al. Protein kinase A activation confers resistance to trastuzumab in human breast cancer cell lines. Clin Cancer Res 15:7196-206 (2009). IHC-P ; Human . Read more (PubMed: 19920112) »

    See all 2 Publications for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab32514.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册