RabMAb

Anti-PKA gamma (catalytic subunit)抗体[EP2647Y] (ab108385)

概述

  • 产品名称
    Anti-PKA gamma (catalytic subunit)抗体[EP2647Y]
    参阅全部 PKA gamma (catalytic subunit) 一抗
  • 描述
    兔单克隆抗体[EP2647Y] to PKA gamma (catalytic subunit)
  • 宿主
    Rabbit
  • 特异性
    This antibody is likely to react with the alpha subunit, as it possesses 85.7% similar homology with the immunogen. Additionally, it may react with the beta subunit, possessing 78.6% homology.
  • 经测试应用
    适用于: WB, IP, Flow Cytmore details
    不适用于: ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of Human PKA gamma (catalytic subunit).

  • 阳性对照
    • HeLa, MCF7, PC3 and A549 cell lysates
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

应用

Our Abpromise guarantee covers the use of ab108385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000 - 1/200000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • 应用说明
    Is unsuitable for ICC/IF or IHC-P.
  • 靶标

    • 功能
      Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
    • 组织特异性
      Testis specific. But important tissues such as brain and ovary have not been analyzed for the content of transcript.
    • 序列相似性
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • cAMP-dependent protein kinase catalytic subunit gamma antibody
      • KAPCG_HUMAN antibody
      • KAPG antibody
      • PKA C gamma antibody
      • PKA C-gamma antibody
      • PKACg antibody
      • PRKACG antibody
      • Protein kinase cAMP dependent catalytic gamma antibody
      • Serine (threonine) protein kinase antibody
      see all

    图片

    • All lanes : Anti-PKA gamma (catalytic subunit) antibody [EP2647Y] (ab108385) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : PC3 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa

    文献

    This product has been referenced in:
    • Liu G  et al. ColXV promotes adipocyte differentiation via inhibiting DNA methylation and cAMP/PKA pathway in mice. Oncotarget 8:60135-60148 (2017). Read more (PubMed: 28947959) »

    See 1 Publication for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T cell line)
    Gel Running Conditions
    Reduced Denaturing (12 % gel)
    Loading amount
    2e+006 cells
    Specification
    HEK293T cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 29 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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