Anti-PKA beta (catalytic subunit)抗体(ab64801)

概述

  • 产品名称Anti-PKA beta (catalytic subunit)抗体
    参阅全部 PKA beta (catalytic subunit) 一抗
  • 描述
    兔多克隆抗体to PKA beta (catalytic subunit)
  • 特异性Detects endogenous levels of total PKA beta (catalytic subunit) protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthetic peptide derived from an internal region of human PKA beta (catalytic subunit)

  • 阳性对照
    • Extracts from Jurkat cells and CoLo205 cells

性能

应用

Our Abpromise guarantee covers the use of ab64801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). Other bands are detected when using extracts from CoLo205 cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
    • 组织特异性Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.
    • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
    • 细胞定位Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • cAMP-dependent protein kinase catalytic beta subunit isoform 4ab antibody
      • cAMP-dependent protein kinase catalytic subunit beta antibody
      • KAPCB_HUMAN antibody
      • PKA C beta antibody
      • PKA C-beta antibody
      • PKACB antibody
      • Prkacb antibody
      • protein kinase A catalytic subunit beta antibody
      • Protein kinase cAMP dependent catalytic beta antibody
      see all

    Anti-PKA beta (catalytic subunit) antibody 图像

    • All lanes : Anti-PKA beta (catalytic subunit) antibody (ab64801) at 1/500 dilution

      Lane 1 : Extracts from Jurkat cells at 5-30ug with no immunizing peptide
      Lane 2 : Extracts from CoLo205 cells at 5-30ug with no immunizing peptide
      Lane 3 : CoLo205 cells at 5-30ug with immunizing peptide at 5-10ug


      Predicted band size : 41 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Anti-PKA beta (catalytic subunit) antibody (ab64801)参考文献

    ab64801 has not yet been referenced specifically in any publications.

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