Anti-PKA alpha + beta (catalytic subunits) (phospho T197)抗体(ab5815)

概述

  • 产品名称Anti-PKA alpha + beta (catalytic subunits) (phospho T197)抗体
    参阅全部 PKA alpha + beta (catalytic subunits) 一抗
  • 描述
    兔多克隆抗体to PKA alpha + beta (catalytic subunits) (phospho T197)
  • 特异性This antibody exibited a preference for PKA catalytic subunit beta in some tested cell lines.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic phosphopeptide derived from a region of human PKA that contains threonine 197.

  • 阳性对照
    • Forskolin-treated NIH3T3 cells, and Y-1 mouse adrenal cortical cells.

性能

应用

Our Abpromise guarantee covers the use of ab5815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.75 µg/ml. Detects a band of approximately 42 kDa.

靶标

  • 相关性PRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
  • 细胞定位Cytoplasm. Nucleus. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm
  • 数据库链接
  • 别名
    • cAMP dependent protein kinase beta catalytic subunit antibody
    • cAMP dependent protein kinase alpha catalytic subunit antibody
    • cAMP dependent protein kinase catalytic subunit alpha antibody
    • cAMP dependent protein kinase catalytic subunit beta antibody
    • PKA C alpha antibody
    • PKA C beta antibody
    • PKACA antibody
    • PKACB antibody
    • PRKACA antibody
    • PRKACB antibody
    • Protein kinase cAMP dependent catalytic alpha antibody
    • Protein kinase cAMP dependent catalytic beta antibody
    see all

Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody 图像

  • Peptide Competition and Phosphatase Treatment: Lysates prepared from Y1 Adrenocortical cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, then incubated with 0.35 µg/mL ab5815 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA [pT197] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab5815)参考文献

This product has been referenced in:
  • Ellison GM  et al. Acute beta-adrenergic overload produces myocyte damage through calcium leakage from the ryanodine receptor 2 but spares cardiac stem cells. J Biol Chem 282:11397-409 (2007). Read more (PubMed: 17237229) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and you have tried many of the suggestions that I would recommend. You have tried various dilutions an...

Read More

Thank you for your enquiry regarding an antiPKA which is specific for PKA catalytic subunit(both the alpha and beta forms)for use in Western blot. The product catalogue number which matches your querie specifications is ab5815. Please refer to the onli...

Read More

I'm afraid I don't know. ab8356 was shown to work in IF by one of our customers, Dr Martha Simpson-Holley . Your customer may contact her via the e-mail link in the reviews section of Abcam's online datasheet to request more details of her protocol. To...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"