Anti-PKA alpha + beta (catalytic subunits)抗体- N-terminal (ab71764)

概述

  • 产品名称Anti-PKA alpha + beta (catalytic subunits)抗体- N-terminal
    参阅全部 PKA alpha + beta (catalytic subunits) 一抗
  • 描述
    兔多克隆抗体to PKA alpha + beta (catalytic subunits) - N-terminal
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from the N-terminal region, between amino acids 10-40, of human PKA alpha + beta (catalytic subunits), conjugated to KLH

  • 阳性对照
    • Jurkat cell and mouse heart tissue lysates and human hepatocarcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab71764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 35 kDa (predicted molecular weight: 41 kDa).

靶标

  • 相关性PRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
  • 细胞定位Cytoplasm. Nucleus. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm
  • 数据库链接
  • 别名
    • cAMP dependent protein kinase beta catalytic subunit antibody
    • cAMP dependent protein kinase alpha catalytic subunit antibody
    • cAMP dependent protein kinase catalytic subunit alpha antibody
    • cAMP dependent protein kinase catalytic subunit beta antibody
    • PKA C alpha antibody
    • PKA C beta antibody
    • PKACA antibody
    • PKACB antibody
    • PRKACA antibody
    • PRKACB antibody
    • Protein kinase cAMP dependent catalytic alpha antibody
    • Protein kinase cAMP dependent catalytic beta antibody
    see all

Anti-PKA alpha + beta (catalytic subunits) antibody - N-terminal 图像

  • All lanes : Anti-PKA alpha + beta (catalytic subunits) antibody - N-terminal (ab71764) at 1/100 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : mouse heart tissue lysate

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 34,35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
  • ab71764 at 1/50 dilution, staining PKA alpha + beta (catalytic subunits) in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

Anti-PKA alpha + beta (catalytic subunits) antibody - N-terminal (ab71764)参考文献

ab71764 has not yet been referenced specifically in any publications.

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