Anti-PKA 2 beta (regulatory subunit) (phospho S114)抗体(ab5829)

概述

  • 产品名称Anti-PKA 2 beta (regulatory subunit) (phospho S114)抗体
    参阅全部 PKA 2 beta (regulatory subunit) 一抗
  • 描述
    兔多克隆抗体to PKA 2 beta (regulatory subunit) (phospho S114)
  • 特异性PKA regulatory 2 alpha subunit (75% homologous) has not been tested.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Cow
  • 免疫原

    Synthetic phosphopeptide derived from a region of human PKA regulatory 2 beta subunit that contains serine 114.

  • 阳性对照
    • 3T3-L1 adipocytes or Y1 mouse adrenal cortical cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKA. The final product is generated by affinity chromatography using a PKA-derived peptide that is phosphorylated at serine 114.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 50 kDa.

靶标

  • 功能Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
  • 组织特异性Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • 序列相似性Belongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • 翻译后修饰Phosphorylated by the activated catalytic chain.
  • 细胞定位Cytoplasm. Cell membrane. Co-localizes with PJA2 in the cytoplasm and at the cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI451071 antibody
    • AW061005 antibody
    • cAMP dependent protein kinase type II beta regulatory chain antibody
    • cAMP dependent protein kinase type II beta regulatory subunit antibody
    • cAMP-dependent protein kinase type II-beta regulatory subunit antibody
    • H RG363E19.2 antibody
    • KAP3_HUMAN antibody
    • MGC116401 antibody
    • Pkarb2 antibody
    • PRKAR 2 antibody
    • PRKAR2 antibody
    • PRKAR2B antibody
    • Protein kinase cAMP dependent regulatory type II beta antibody
    • RATDNA antibody
    • RII beta antibody
    • RII(beta) antibody
    • RIIbeta antibody
    • WUGSC:H RG363E19.2 antibody
    see all

Anti-PKA 2 beta (regulatory subunit) (phospho S114) antibody 图像

  • Peptide Competition and Phosphatase Treatment: Lysates prepared from 3T3-L1 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, and incubated with ab5829 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA reg 2 beta [pS114] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

Anti-PKA 2 beta (regulatory subunit) (phospho S114) antibody (ab5829)参考文献

ab5829 has not yet been referenced specifically in any publications.

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