概述

  • 产品名称Anti-PIWIL4抗体
    参阅全部 PIWIL4 一抗
  • 描述
    兔多克隆抗体to PIWIL4
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse PIWIL4.

    (Peptide available as ab23535.)

  • 阳性对照
    • Mouse testis lysate This antibody gave a positive signal in the following Formaldehyde fixed cell line: HeLa.

性能

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应用

Our Abpromise guarantee covers the use of ab21869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). Block with 5% BSA for 1 hour and incubate antibody in TBST for 1 hour or more.

靶标

  • 功能Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
  • 组织特异性Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.
  • 序列相似性Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 翻译后修饰Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • 细胞定位Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686P01248 antibody
    • FLJ36156 antibody
    • HILI 2 antibody
    • HILI2 antibody
    • HIWI 2 antibody
    • HIWI2 antibody
    • Miwi 2 protein antibody
    • Miwi2 antibody
    • PIWI antibody
    • Piwi like 2 antibody
    • Piwi like 4 (Drosophila) antibody
    • Piwi like 4 antibody
    • Piwi like protein 4 antibody
    • PIWI like protein antibody
    • Piwi like RNA mediated gene silencing 4 antibody
    • Piwi-like protein 4 antibody
    • PIWIL 4 antibody
    • Piwil4 antibody
    • PIWL4_HUMAN antibody
    see all

Anti-PIWIL4 antibody 图像

  • Lanes 1 - 2 : Anti-PIWIL4 antibody (ab21869) at 2 µg/ml
    Lanes 3 - 4 : Anti-PIWIL4 antibody (ab21869) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
    Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with Mouse PIWIL4 peptide (ab23535) at 1 µg/ml
    Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
    Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with Mouse PIWIL4 peptide (ab23535) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 98 kDa
    Observed band size : 98 kDa

    See Abreview

  • ICC/IF image of ab21869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab21869 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PIWIL4 antibody (ab21869)参考文献

This product has been referenced in:
  • Keam SP  et al. The human Piwi protein Hiwi2 associates with tRNA-derived piRNAs in somatic cells. Nucleic Acids Res 42:8984-95 (2014). WB, ICC/IF ; Human . Read more (PubMed: 25038252) »
  • Yabuta Y  et al. TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice. J Cell Biol 192:781-95 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21383078) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (mouse ES cells)
Total protein in input 100 µg
Immuno-precipitation step Protein A/G
Specification mouse ES cells
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提交于 Mar 20 2014

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (Gradient Gel)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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提交于 Mar 14 2014

Thank you for your message and for providing this further information. I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange ...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for mo...

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Thank you for your enquiry. The mice for catalog number ab4027, the product used in the Western blot on the datasheet for ab21869, are adults, approximately 3 months old. Please let me know if you have any more questions.

I'm sorry to hear you are having a problem with ab21869. This antibody has been tested in mouse samples and if your testis cell extract are from mice I would expect ab21869 to work well for you. I would like to suggest the following modifications t...

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