概述

  • 产品名称Anti-PIWIL2抗体
    参阅全部 PIWIL2 一抗
  • 描述
    兔多克隆抗体to PIWIL2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    VITRYNNRTYRIDDVD

    , corresponding to internal sequence amino acids 418-433 of Human PIWIL2

  • 阳性对照
    • Testis tissue. Human fetal testis lysate.

性能

应用

Our Abpromise guarantee covers the use of ab85084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/500. Predicted molecular weight: 110 kDa.
IHC-P 1/100 - 1/400.

靶标

  • 功能Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
  • 组织特异性Expressed in adult testis and in most tumors.
  • 序列相似性Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 翻译后修饰Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
  • 细胞定位Cytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancer/testis antigen 80 antibody
    • CT80 antibody
    • Hiwi like antibody
    • MILI antibody
    • Miwi like antibody
    • Piwi like 2 antibody
    • Piwi-like protein 2 antibody
    • Piwil2 antibody
    • PIWL2_HUMAN antibody
    see all

Anti-PIWIL2 antibody 图像

  • Immunohistochemistry analysis of PIWIL2 in formalin-fixed paraffin-embedded human fetal testis tissue sections using ab85084 at 1/200 dilution.

  • Anti-PIWIL2 antibody (ab85084) at 1/200 dilution + Human fetal testis lysate

    Predicted band size : 110 kDa
    Observed band size : 110 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-PIWIL2 antibody (ab85084)参考文献

This product has been referenced in:
  • Berdasco M  et al. DNA Methylation Plasticity of Human Adipose-Derived Stem Cells in Lineage Commitment. Am J Pathol : (2012). WB . Read more (PubMed: 23031258) »

See 1 Publication for this product

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