概述

  • 产品名称Anti-PIWIL2抗体
    参阅全部 PIWIL2 一抗
  • 描述
    兔多克隆抗体to PIWIL2
  • 经测试应用适用于: IHC-Fr, WB, Flow Cyt, ICC/IF, IP, Electron Microscopymore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse MILI/ HILI.

    (Peptide available as ab27821.)

  • 阳性对照
    • Mouse Testis Whole Tissue Lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab36764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 20534472
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).Can be blocked with Mouse PIWIL2 peptide (ab27821).
Flow Cyt Use at an assay dependent concentration. PubMed: 20460113ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 20460113
IP Use at an assay dependent concentration. PubMed: 20460113
Electron Microscopy Use at an assay dependent concentration. PubMed: 20011505

靶标

  • 功能Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
  • 组织特异性Expressed in adult testis and in most tumors.
  • 序列相似性Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 翻译后修饰Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
  • 细胞定位Cytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancer/testis antigen 80 antibody
    • CT80 antibody
    • Hiwi like antibody
    • MILI antibody
    • Miwi like antibody
    • Piwi like 2 antibody
    • Piwi-like protein 2 antibody
    • Piwil2 antibody
    • PIWL2_HUMAN antibody
    see all

Anti-PIWIL2 antibody 图像

  • Anti-PIWIL2 antibody (ab36764) at 1 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ab36764 staining PIWIL2 in murine testis tissue by Immunohistochemistry (Frozen sections). Chromatin was stained with DAPI.

Anti-PIWIL2 antibody (ab36764)参考文献

This product has been referenced in:
  • Zhou J  et al. STK31/TDRD8, a germ cell-specific factor, is dispensable for reproduction in mice. PLoS One 9:e89471 (2014). IF ; Mouse . Read more (PubMed: 24586802) »
  • Castañeda J  et al. Reduced pachytene piRNAs and translation underlie spermiogenic arrest in Maelstrom mutant mice. EMBO J N/A:N/A (2014). Mouse . Read more (PubMed: 25063675) »

See all 8 Publications for this product

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The Western Blot image was obtained using 1 µg/ml of ab36764.

The IHC-Fr image comes from a publication : http://www.ncbi.nlm.nih.gov/pubmed/20534472?dopt=Abstract. Unfortunately there is no infor...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Testis)
Loading amount 30 µg
Specification Testis
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Feb 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"