概述

  • 产品名称Anti-PIWIL1抗体
    参阅全部 PIWIL1 一抗
  • 描述
    小鼠多克隆抗体to PIWIL1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human PIWIL1 protein (NP_004755)

  • 阳性对照
    • HepG2 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab89427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.

靶标

  • 功能Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.
  • 组织特异性Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells.
  • 序列相似性Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 结构域The PAZ domain specifically recognizes binds the 2'-O-methylated 3'-end of piRNAs.
  • 翻译后修饰Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • 细胞定位Cytoplasm. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Also present in chromatoid body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HIWI antibody
    • MIWI antibody
    • Piwi (Drosophila) like 1 antibody
    • PIWI antibody
    • Piwi homolog antibody
    • Piwi like 1 (drosophila) antibody
    • Piwi like 1 antibody
    • Piwi-like protein 1 antibody
    • PIWIL1 antibody
    • PIWL1_HUMAN antibody
    see all

Anti-PIWIL1 antibody 图像

  • Anti-PIWIL1 antibody (ab89427) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 99 kDa

Anti-PIWIL1 antibody (ab89427)参考文献

This product has been referenced in:
  • Xu K  et al. AKAP3 synthesis is mediated by RNA binding proteins and PKA signaling during mouse spermiogenesis. Biol Reprod 90:119 (2014). Read more (PubMed: 24648398) »

See 1 Publication for this product

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