概述

  • 产品名称Anti-PIWIL1抗体
    参阅全部 PIWIL1 一抗
  • 描述
    兔多克隆抗体to PIWIL1
  • 特异性At least three isoforms of PIWIL1 are known to exist; ab105393 will recognize the two longest isoforms. ab105393 is predicted to not cross-react with other PIWI family members.
  • 经测试应用适用于: IHC-P, ICC/IF, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    18 amino acid synthetic peptide near the N-terminus of Human PIWIL1 (AAK69348).

  • 阳性对照
    • HepG2 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab105393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 99 kDa.
ICC Use a concentration of 10 µg/ml.

靶标

  • 功能Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.
  • 组织特异性Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells.
  • 序列相似性Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 结构域The PAZ domain specifically recognizes binds the 2'-O-methylated 3'-end of piRNAs.
  • 翻译后修饰Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • 细胞定位Cytoplasm. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Also present in chromatoid body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HIWI antibody
    • MIWI antibody
    • Piwi (Drosophila) like 1 antibody
    • PIWI antibody
    • Piwi homolog antibody
    • Piwi like 1 (drosophila) antibody
    • Piwi like 1 antibody
    • Piwi-like protein 1 antibody
    • PIWIL1 antibody
    • PIWL1_HUMAN antibody
    see all

Anti-PIWIL1 antibody 图像

  • Immunofluorescence of PIWIL1 in rat liver tissue with ab105393 at 20 ug/mL.
  • All lanes : Anti-PIWIL1 antibody (ab105393) at 1 µg/ml

    Lane 1 : HepG2 cell lysate without blocking peptide.
    Lane 2 : HepG2 cell lysate with blocking peptide.

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 99 kDa
  • Immunohistochemical analysis of rat liver tissue, staining PIWIL1 with ab105393 at 10 µg/ml.

Anti-PIWIL1 antibody (ab105393)参考文献

This product has been referenced in:
  • Chen Z  et al. Stem cell protein Piwil1 endowed endometrial cancer cells with stem-like properties via inducing epithelial-mesenchymal transition. BMC Cancer 15:811 (2015). IHC . Read more (PubMed: 26506848) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with Tween
Specification Breast
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

提交于 Mar 08 2016

Abreviews
Application RIP
Sample Cow Cell lysate - whole cell (Testis)
Negative control 2 µg non-specific rabbit IgG (Milipore, #12-370)
Immuno-precipitation step Protein A
Specification Testis
Detection step Other
Type RIP without cross-linking
Duration of cross-linking step: 0 second(s)
Positive control Testis with 3 µg Ab105393 Anti-PIWIL1
Username

Dr. Stewart Russell

Verified customer

提交于 Oct 16 2015

Application Western blot
Sample Cow Tissue lysate - whole (Testis)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification Testis
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Stewart Russell

Verified customer

提交于 Jul 14 2015

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The sequence homology between human and Platypus species seems quite OK for antibodies ab12337 and ab85084 cross reactivity. These antibodies may be tried for further tests. We can provide abrreview discount for th...

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