Anti-PITX2抗体(ab32832)

概述

  • 产品名称Anti-PITX2抗体
    参阅全部 PITX2 一抗
  • 描述
    兔多克隆抗体to PITX2
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: SVLAPGQPRS LDSSKHRLEV HTISDTSSPE AAEKDKSQQG KNEDVGAEDP, corresponding to a region within N terminal amino acids 37-86 of Human PITX2

  • 阳性对照
    • HepG2 cell lysate. Human intestine.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/62500.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能May play an important role in development and maintenance of anterior structures. Isoform PTX2C is involved in left-right asymmetry the developing embryo.
  • 疾病相关Defects in PITX2 are the cause of Axenfeld-Rieger syndrome type 1 (RIEG1) [MIM:180500]; also known as Rieger syndrome type 1. RIEG1 is an autosomal dominant defect characterized by hypodontia (partial anodontia), anal stenosis, hypertelorism, mental deficiency, agenesis of the facial bones, with malformation of the anterior chamber of the eye.
    Defects in PITX2 are the cause of iridogoniodysgenesis type 2 (IRID2) [MIM:137600]; also known as iridogoniodysgenesis syndrome 2 (IGDS2). It is an autosomal dominant inherited disease.
    Defects in PITX2 are a cause of Peters anomaly (PAN) [MIM:604229]. It is a congenital defect of the anterior chamber of the eye.
    Defects in PITX2 are associated with ring dermoid of cornea (RDC) [MIM:180550]. RDC is an autosomal dominantly inherited syndrome characterized by bilateral annular limbal dermoids with corneal and conjunctival extension.
  • 序列相似性Belongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • All1 responsive gene 1 antibody
    • ALL1 responsive protein ARP1 antibody
    • ALL1-responsive protein ARP1 antibody
    • ARP 1 antibody
    • ARP1 antibody
    • Brx 1 antibody
    • Brx1 antibody
    • Homeobox protein PITX2 antibody
    • IDG 2 antibody
    • IDG2 antibody
    • IGDS 2 antibody
    • IGDS antibody
    • IGDS2 antibody
    • IHG 2 antibody
    • IHG2 antibody
    • IRID 2 antibody
    • IRID2 antibody
    • MGC111022 antibody
    • MGC20144 antibody
    • Otlx 2 antibody
    • Otlx2 antibody
    • Paired like homeodomain transcription factor 2 antibody
    • Paired-like homeodomain transcription factor 2 antibody
    • Pituitary homeo box 2 antibody
    • Pituitary homeobox 2 antibody
    • PITX 2 antibody
    • pitx2 antibody
    • PITX2_HUMAN antibody
    • PTX 2 antibody
    • PTX2 antibody
    • RGS antibody
    • RIEG 1 antibody
    • RIEG antibody
    • Rieg bicoid related homeobox transcription factor 1 antibody
    • RIEG bicoid related homeobox transcription factor antibody
    • RIEG bicoid-related homeobox transcription factor antibody
    • RIEG1 antibody
    • RS antibody
    • Solurshin antibody
    see all

Anti-PITX2 antibody 图像

  • Anti-PITX2 antibody (ab32832) at 2.5 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling PITX2 with ab32832 at 4-8µg/ml. Cells with positive label (as indicated by the arrows) are epithelial cells of the intestinal villus.

Anti-PITX2 antibody (ab32832)参考文献

ab32832 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chicken Tissue sections (Neural tube)
Specification Neural tube
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

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Verified customer

提交于 Dec 21 2011

DISCOUNT CODE: *** Expiration date: January 31, 2012 I am pleased to hear you would like to test ab32832 in chicken tissue. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abrev...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Skeletal muscle)
Loading amount 10 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 May 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"