概述

  • 产品名称Anti-PITX1抗体
    参阅全部 PITX1 一抗
  • 描述
    兔多克隆抗体to PITX1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Chimpanzee, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a sequence between N terminal amino acids 50-100 of Human PITX1. (NP_002644.3)

  • 阳性对照
    • HeLa whole cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2500 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
IP Use at 1-4 µg/mg of lysate.

靶标

  • 功能May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
  • 疾病相关Defects in PITX1 are a cause of congenital clubfoot (CCF) [MIM:119800]; also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait.
  • 序列相似性Belongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BFT antibody
    • CCF antibody
    • Hindlimb expressed homeobox protein backfoot antibody
    • Hindlimb-expressed homeobox protein backfoot antibody
    • Homeobox protein PITX1 antibody
    • LBNBG antibody
    • Paired like homeodomain 1 antibody
    • Paired like homeodomain transcription factor 1 antibody
    • Paired-like homeodomain transcription factor 1 antibody
    • Pituitary homeo box 1 antibody
    • Pituitary homeobox 1 antibody
    • Pituitary otx related factor antibody
    • Pitx1 antibody
    • PITX1_HUMAN antibody
    • POTX antibody
    • PTX1 antibody
    see all

Anti-PITX1 antibody 图像

  • HeLa whole cell lysate (1 mg) was immunoprecipitated using ab70273 at 3 µg/mg lysate (lane 1) or an IgG control (lane 2). One quarter of the total IP reaction was loaded on to a gel and analysed by Western blot using ab70273 at 1 µg/ml. Bands were detected using chemiluminescence with an exposure time of 30 seconds.
  • All lanes : Anti-PITX1 antibody (ab70273) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 34 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

Anti-PITX1 antibody (ab70273)参考文献

This product has been referenced in:
  • Booy EP  et al. The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor PITX1. Nucleic Acids Res 42:3346-61 (2014). Human . Read more (PubMed: 24369427) »

See 1 Publication for this product

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