概述

  • 产品名称Anti-PITRM1抗体
    参阅全部 PITRM1 一抗
  • 描述
    兔多克隆抗体to PITRM1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 1-188 of Human PITRM1 (AAH01150).

  • 阳性对照
    • A549, HeLa, HepG2 and HCT116 cells and whole cell lysates; PC13 xenograft tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab111681 is purified by antigen-affinity chromatography.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 117 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

靶标

  • 功能ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.
  • 组织特异性Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta.
  • 序列相似性Belongs to the peptidase M16 family. PreP subfamily.
  • 翻译后修饰The disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hMP1 antibody
    • hPreP antibody
    • KIAA1104 antibody
    • Metalloprotease 1 antibody
    • MGC138192 antibody
    • MGC141929 antibody
    • mitochondrial antibody
    • MP1 antibody
    • Pitrilysin metallopeptidase 1 antibody
    • Pitrilysin metalloproteinase 1 antibody
    • PITRM 1 antibody
    • PITRM1 antibody
    • PreP antibody
    • PreP peptidasome antibody
    • PREP_HUMAN antibody
    • Presequence protease antibody
    • Presequence protease mitochondrial antibody
    see all

Anti-PITRM1 antibody 图像

  • Anti-PITRM1 antibody (ab111681) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 117 kDa
  • ab111681 at 1/200 dilution staining PITRM1 in methanol-fixed HeLa cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
  • ab111681 at 1/500 dilution staining PITRM1 in paraffin-embedded PC13 xenograft tissue by Immunohistochemistry.

Anti-PITRM1 antibody (ab111681)参考文献

ab111681 has not yet been referenced specifically in any publications.

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