The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
Note=A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
Contains 1 PDZ (DHR) domain.
The PDZ domain mediates interactions with FZD5, FZD8, ACCN3, GRID2, CLCN3 (By similarity). Mediates also interaction with CFTR and ADRB1. The coiled-coil region probably mediates association to membranes, targeting to the Golgi, and interactions with GOLGA3, and STX6. May also mediate interaction with RHOQ.
Cytoplasm. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Enriched in synaptosomal and postsynaptic densities (PSD) fractions (By similarity). Expressed in cell bodies and dendrites of Purkinje cells (By similarity). Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.
Golgi associated PDZ and coiled coil motif containing antibody
Golgi associated PDZ and coiled coil motif containing protein antibody
Golgi-associated PDZ and coiled-coil motif-containing protein antibody
GOPC 1 antibody
PDZ protein interacting specifically with TC 10 antibody
PDZ protein interacting specifically with TC10 antibody
PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody
PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody
Protein interacting specifically with Tc 10 antibody
Protein interacting specifically with Tc10 antibody
Immunoprecipitation - PIST antibody (ab95067)
ab95067 detecting PIST in HeLa whole cell lysate by WB following IP.
Lane 1: ab95067 at 3 µg/mg of lysate
Lane 2: IP with an antibody which recognizes a downstream epitope of PIST
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds.