概述

  • 产品名称
  • 描述
    兔多克隆抗体to Pirin
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: GEPLREPVIQ HGPFVMNTNE EISQAILDFR NAKNGFERAK TWKSKIGN, corresponding to amino acids 243-290 of Human Pirin

  • 阳性对照
    • HepG2 cell lystes and Human lung tissue.

性能

应用

Our Abpromise guarantee covers the use of ab55978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Possible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro).
  • 组织特异性
    Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.
  • 通路
    Flavonoid metabolism; quercetin degradation.
  • 序列相似性
    Belongs to the pirin family.
  • 细胞定位
    Nucleus. Cytoplasm. Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3-dioxygenase PIR antibody
    • Iron binding nuclear protein antibody
    • Pir antibody
    • PIR_HUMAN antibody
    • Pirin antibody
    • Probable quercetin 2 antibody
    • probable quercetin 2,3-dioxygenase PIR antibody
    • Probable quercetinase antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Pirin with ab55978 at 4-8µg/ml. Arrow indicate postively labelled alveolar cells. Magnification: 400X.

  • Anti-Pirin antibody (ab55978) at 1.25 µg/ml + HepG2 cell lyste at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa

文献

ab55978 has not yet been referenced specifically in any publications.

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