概述

  • 产品名称
  • 描述
    山羊多克隆抗体to Pirin
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    GSSKKVTLSVLSREC

    , corresponding to N terminal amino acids 2-15 of Human Pirin.

  • 阳性对照
    • Human heart lysate. Lysate prepared from transfected HEK293 transiently expressing Pirin.

性能

应用

Our Abpromise guarantee covers the use of ab21202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 32 kDa).

靶标

  • 功能
    Possible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro).
  • 组织特异性
    Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.
  • 通路
    Flavonoid metabolism; quercetin degradation.
  • 序列相似性
    Belongs to the pirin family.
  • 细胞定位
    Nucleus. Cytoplasm. Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3-dioxygenase PIR antibody
    • Iron binding nuclear protein antibody
    • Pir antibody
    • PIR_HUMAN antibody
    • Pirin antibody
    • Probable quercetin 2 antibody
    • probable quercetin 2,3-dioxygenase PIR antibody
    • Probable quercetinase antibody
    see all

Anti-Pirin antibody 图像

  • Anti-Pirin antibody (ab21202) at 0.3 µg/ml + Human Heart lysate (35µg protein in RIPA buffer).
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 30-35 kDa (why is the actual band size different from the predicted?)
  • Anti-Pirin antibody (ab21202) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently over-expressing Pirin.

    Predicted band size : 32 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-Pirin antibody (ab21202)参考文献

This product has been referenced in:
  • Brzóska K  et al. Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element. Mol Cell Biochem 389:99-111 (2014). WB ; Human . Read more (PubMed: 24390086) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab21202.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册