Anti-PIP5K3 (phospho S307)抗体(ab62467)

概述

  • 产品名称Anti-PIP5K3 (phospho S307)抗体
    参阅全部 PIP5K3 一抗
  • 描述
    兔多克隆抗体to PIP5K3 (phospho S307)
  • 特异性ab62467 detects endogenous levels of PIP5K3 only when phosphorylated at serine 307.
  • 经测试应用适用于: ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human PIP5K3 around the phosphorylation site of serine 307 (S-A-SP-I-T).

  • 阳性对照
    • Human brain tissue and HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab62467 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes.
  • 疾病相关Corneal dystrophy, fleck
  • 序列相似性Contains 1 DEP domain.
    Contains 1 FYVE-type zinc finger.
    Contains 1 PIPK domain.
  • 细胞定位Endosome membrane. Mainly associated with membranes of the late endocytic pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 phosphatidylinositol 3 phosphate 5 kinase antibody
    • 1 phosphatidylinositol 4 phosphate 5 kinase antibody
    • 1-phosphatidylinositol 3-phosphate 5-kinase antibody
    • CFD antibody
    • epididymis luminal protein 37 antibody
    • FAB1 antibody
    • FAB1, S. cerevisiae, homolog of antibody
    • FYV1_HUMAN antibody
    • FYVE finger containing phosphoinositide kinase antibody
    • FYVE finger-containing phosphoinositide kinase antibody
    • KIAA0981 antibody
    • MGC40423 antibody
    • p235 antibody
    • Phosphatidylinositol 3 phosphate 5 kinase type III antibody
    • Phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase type III antibody
    • Phosphatidylinositol 3-phosphate 5-kinase antibody
    • Phosphatidylinositol 3-phosphate 5-kinase type III antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type III antibody
    • Phosphoinositide kinase, FYVE finger containing antibody
    • PIKfyve antibody
    • PIP5K antibody
    • PIP5K3 antibody
    • PIPkin III antibody
    • PIPkin-III antibody
    • PtdIns(4)P 5 kinase antibody
    • Type III PIP kinase antibody
    • ZFYVE29 antibody
    • Zinc finger, FYVE domain containing 29 antibody
    see all

Anti-PIP5K3 (phospho S307) antibody 图像

  • ab62467, at 1/50 dilution, staining PIP5K3 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
  • ab62467, at 1/500 dilution, staining PIP5K3 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-PIP5K3 (phospho S307) antibody (ab62467)参考文献

ab62467 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Mammary Epithelial)
Loading amount 50 µg
Specification Mammary Epithelial
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step Odyssey Blocking Buffer (Li-COR) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30%
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提交于 Jul 08 2011

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