Anti-PIP5K3 (phospho S307)抗体(ab62467)

概述

  • 产品名称
    Anti-PIP5K3 (phospho S307)抗体
    参阅全部 PIP5K3 一抗
  • 描述
    兔多克隆抗体to PIP5K3 (phospho S307)
  • 特异性
    ab62467 detects endogenous levels of PIP5K3 only when phosphorylated at serine 307.
  • 经测试应用
    适用于: ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human PIP5K3 around the phosphorylation site of serine 307 (S-A-SP-I-T).

  • 阳性对照
    • Human brain tissue and HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab62467 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能
    The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes.
  • 疾病相关
    Corneal dystrophy, fleck
  • 序列相似性
    Contains 1 DEP domain.
    Contains 1 FYVE-type zinc finger.
    Contains 1 PIPK domain.
  • 细胞定位
    Endosome membrane. Mainly associated with membranes of the late endocytic pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 phosphatidylinositol 3 phosphate 5 kinase antibody
    • 1 phosphatidylinositol 4 phosphate 5 kinase antibody
    • 1-phosphatidylinositol 3-phosphate 5-kinase antibody
    • CFD antibody
    • epididymis luminal protein 37 antibody
    • FAB1 antibody
    • FAB1, S. cerevisiae, homolog of antibody
    • FYV1_HUMAN antibody
    • FYVE finger containing phosphoinositide kinase antibody
    • FYVE finger-containing phosphoinositide kinase antibody
    • KIAA0981 antibody
    • MGC40423 antibody
    • p235 antibody
    • Phosphatidylinositol 3 phosphate 5 kinase type III antibody
    • Phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase type III antibody
    • Phosphatidylinositol 3-phosphate 5-kinase antibody
    • Phosphatidylinositol 3-phosphate 5-kinase type III antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type III antibody
    • Phosphoinositide kinase, FYVE finger containing antibody
    • PIKfyve antibody
    • PIP5K antibody
    • PIP5K3 antibody
    • PIPkin III antibody
    • PIPkin-III antibody
    • PtdIns(4)P 5 kinase antibody
    • Type III PIP kinase antibody
    • ZFYVE29 antibody
    • Zinc finger, FYVE domain containing 29 antibody
    see all

图片

  • ab62467, at 1/50 dilution, staining PIP5K3 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
  • ab62467, at 1/500 dilution, staining PIP5K3 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

文献

ab62467 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Mammary Epithelial)
Loading amount
50 µg
Specification
Mammary Epithelial
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Odyssey Blocking Buffer (Li-COR) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30%
Username

Abcam user community

Verified customer

提交于 Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册