Anti-PIP5K1 alpha抗体(ab5466)

概述

  • 产品名称Anti-PIP5K1 alpha抗体
    参阅全部 PIP5K1 alpha 一抗
  • 描述
    兔多克隆抗体to PIP5K1 alpha
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 34-50 of Human PIP5K1 A.

  • 阳性对照
    • HeLa cell lysate.
  • 常规说明


    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Primary antibody说明Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 61 kDa (predicted molecular weight: 65 kDa).

靶标

  • 功能Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Contributes to the activation of PLD2.
  • 组织特异性Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
  • 序列相似性Contains 1 PIPK domain.
  • 细胞定位Cell membrane. Endomembrane system. Golgi apparatus > Golgi stack. Nucleus speckle. Associated with the plasma membrane and with internal membranes. Associated with Golgi stacks (By similarity). Detected on RAC1-induced plasma membrane ruffles, and on membrane ruffles induced by platelet-derived growth factor. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type I alpha antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type I alpha antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha antibody
    • PI4P5K Ia antibody
    • PI51A_HUMAN antibody
    • PIP5K A antibody
    • PIP5K1-alpha antibody
    • Pip5k1a antibody
    • PIP5KIalpha antibody
    • PtdIns(4)P-5-kinase 1 alpha antibody
    see all

Anti-PIP5K1 alpha antibody 图像



  • Predicted band size : 65 kDa


    ab5466 at a 1/100 dilution staining approximately 61kDa band of PIP5K 1A in HeLa cell lysate by Western blot (ECL).

Anti-PIP5K1 alpha antibody (ab5466)参考文献

ab5466 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. As stated on the online datasheet for this antibody, the immunogen used was human PIP5K1 alpha. The antibody has not yet been tested in mouse. I hope this information helps, please do not hesitate to contact us if yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"