Interaction protein for cytohesin exchange factors 1 antibody
Interactor protein for cytohesin exchange factors 1 antibody
Phosphoinositide binding protein PIP3 E antibody
Phosphoinositide binding protein PIP3E antibody
Phosphoinositide-binding protein PIP3-E antibody
Western blot - Anti-PIP3E antibody (ab80817)
Anti-PIP3E antibody (ab80817) at 1 µg/ml + 721B cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa Observed band size: 49 kDa Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
Immunocytochemistry/ Immunofluorescence - Anti-PIP3E antibody (ab80817)This image is courtesy of an Abreview submitted by Skyla Carney
ab80817 staining PIP3E in human LNCaP Androgen Dependent Prostate cells treated with EGF for 24 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/50 in PBS + 1% BSA) for 24 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/250) was used as the secondary antibody.