The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
TBS buffer is ideal.
Phosphatidylinositol 4,5-biphosphate (PIP2) is a membrane phospholipid that has been implicated in a variety of cellular processes, including synaptic vesicle recycling and signal transduction pathways. PLCD4 hydrolyzes PIP2 to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3).
Anti-PIP2 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-PIP2 antibody (ab2335)Image from Brzeska H et al., J Biol Chem. 2012 Apr 27;287(18):14923-36. Epub 2012 Feb 24. Fig 1.; doi: 10.1074/jbc.M111.318667; April 27, 2012, The Journal of Biological Chemistry, 287, 14923-14936.
ab2335, staining PIP2 in non-polarized (left) and polarized (right) AX3 cells by Immunocytochemisry/ Immunofluorescence.
Cells were fixed with formaldehyde, glutaraldehyde, and 0.01% Triton X-100 in PBS for 10 min. Samples were incubated with primary antibody at 1/100 dilution.
Li J et al. Actin dynamics is rapidly regulated by the PTEN and PIP2 signaling pathways leading to myocyte hypertrophy. Am J Physiol Heart Circ Physiol307:H1618-25 (2014).
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