概述

  • 产品名称Anti-PINX1抗体
    参阅全部 PINX1 一抗
  • 描述
    山羊多克隆抗体to PINX1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide: SMLAERRRKQKWAV, corresponding to N terminal amino acids 2-15 of Human PINX1.

  • 常规说明Principal Names – PINX1; PIN2-interacting protein 1; LPTL; LPTS; MGC8850; FLJ20565; 67-11-3 protein; hepatocellular carcinoma-related putative tumor suppressor. Official Gene Symbol - PINX1 (interim symbol). GenBank Accession Number – NP_060354. LocusLink ID - 54984; 72400 (mouse). Please note: We have been contacted by a researcher working with PINX1 to advise that recent research has shown that there are two splice variants of PINX1, one at 45 kDa (full lenth) and the other at 20 kDa (C-terminus truncated). This antibody detects a band at 37kDa in Jurkat Lysate. The researcher is currently testing this antibody to confirm that the band we find at 37 kDa is infact PINX1. We would be interested to hear back from any other researchers working with PINX1. 11 March, 2004.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab2344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明Western Blot – Approx 35kDa bands seen in HeLa lysate (predicted molecular weight: 37 kDa).
    Recommended for use at 1-3µg/ml.
  • 靶标

    • 功能Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor.
    • 组织特异性Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.
    • 序列相似性Belongs to the PINX1 family.
      Contains 1 G-patch domain.
    • 结构域The TID (telomerase inhibiting domain) domain is sufficient to bind TERT and inhibit its activity.
      The TBM domain mediates interaction with TERF1.
    • 细胞定位Nucleus. Nucleus > nucleolus. Chromosome > telomere. Chromosome > centromere > kinetochore. Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 67-11-3 protein antibody
      • FLJ20565 antibody
      • Hepatocellular carcinoma-related putative tumor suppressor antibody
      • Liver-related putative tumor suppressor antibody
      • LPTL antibody
      • LPTS antibody
      • MGC8850 antibody
      • OTTHUMP00000224984 antibody
      • OTTHUMP00000224985 antibody
      • OTTHUMP00000224986 antibody
      • OTTHUMP00000225053 antibody
      • OTTHUMP00000225054 antibody
      • PIN2 interacting protein 1 antibody
      • Pin2-interacting protein X1 antibody
      • PIN2/TERF1-interacting telomerase inhibitor 1 antibody
      • PINX1 antibody
      • PINX1_HUMAN antibody
      • Protein 67-11-3 antibody
      • TRF1-interacting protein 1 antibody
      see all

    Anti-PINX1 antibody 图像



    • Predicted band size : 37 kDa


      ab2344 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    Anti-PINX1 antibody (ab2344)参考文献

    This product has been referenced in:
    • Herrmann G  et al. Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism. Nucleic Acids Res 35:2321-32 (2007). Read more (PubMed: 17389648) »

    See 1 Publication for this product

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    This antibody has not been tested in IHC. This means that we have not tested it but it does not mean that it will not work in this application. We have no further information on this. If you still wish to purchase the antibody there is a detailed order...

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    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in immunohistochemistry . All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how yo...

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