概述

  • 产品名称Anti-PINK1抗体
    参阅全部 PINK1 一抗
  • 描述
    兔多克隆抗体to PINK1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    LVRALLQREA SKRPSARVAA N

    , corresponding to amino acids 484-504 of Human PINK1.

  • 阳性对照
    • Human, mouse, rat liver.

性能

应用

Our Abpromise guarantee covers the use of ab23707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/1000. Detects a band of approximately 66 kDa.Can be blocked with Human PINK1 peptide (ab30903). Detects a band of approximately 66 kDa. Can be blocked with PINK1 peptide (484-504) (ab30903).
IHC-P Use a concentration of 4 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
  • 组织特异性Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
  • 疾病相关Defects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Mitochondrion outer membrane. Cytoplasm > cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRPK antibody
    • FLJ27236 antibody
    • mitochondrial antibody
    • PARK 6 antibody
    • PARK6 antibody
    • Phosphatase and Tensin Homolog antibody
    • PINK 1 antibody
    • PINK1 antibody
    • PINK1_HUMAN antibody
    • Protein kinase BRPK antibody
    • PTEN induced putative kinase 1 antibody
    • PTEN induced putative kinase protein 1 antibody
    • PTEN-induced putative kinase protein 1 antibody
    • Serine/threonine kinase PINK1 mitochondrial antibody
    • Serine/threonine protein kinase PINK1 mitochondrial antibody
    • Serine/threonine-protein kinase PINK1 antibody
    see all

Anti-PINK1 antibody 图像

  • Anti-PINK1 antibody (ab23707) at 4 µg/ml + Murine liver 100,000 x g pellet at 30 µg

    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa (possible cleavage fragment).
  • ICC/IF image of ab23707 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PINK1 antibody (ab23707) at 1/1000 dilution + Whole cell lysate prepared from Jurkat cells at 100000 cells

    Secondary
    Donkey anti-rabbit IgG conjugated to HRP at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-PINK1 antibody (ab23707)参考文献

This product has been referenced in:
  • Ma L  et al. Restoring Pharmacologic Preconditioning in the Aging Heart: Role of Mitophagy/Autophagy. J Gerontol A Biol Sci Med Sci N/A:N/A (2016). Read more (PubMed: 27565512) »
  • Roe ND  et al. Targeted deletion of PTEN in cardiomyocytes renders cardiac contractile dysfunction through interruption of Pink1-AMPK signaling and autophagy. Biochim Biophys Acta 1852:290-8 (2015). Read more (PubMed: 25229693) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - Triton x-100, 0.01%
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Formaldehyde
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提交于 Jun 19 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - Triton x-100, 0.01%
Fixative Paraformaldehyde
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提交于 Mar 30 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - nuclear (Cardiomyocytes)
Specification Cardiomyocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Mar 02 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Sample Mouse Cell (Cardiomyocytes)
Specification Cardiomyocytes
Permeabilization Yes - Triton x-100, 0.01%
Fixative Formaldehyde
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提交于 Feb 20 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Rat Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Dec 04 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Dec 04 2014



I have contacted the laboratories who have provided the IHC-P testing protocol which I hope will be helpful. Please note this would be a guideline only and may require individual optimization. I have copied it below.




I...

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Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step No blocking step used for 30 minute(s) · Concentration: 4% · Temperature: 16°C
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提交于 Apr 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (SH-SY5Y human neuroblastoma)
Specification SH-SY5Y human neuroblastoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Sep 23 2013

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Application Western blot
Sample Human Cell lysate - whole cell (lung)
Loading amount 15 µg
Specification lung
Gel Running Conditions Reduced Denaturing (12% SDS page)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Apr 23 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"