Anti-PINK1抗体[38CT18.7] (ab75487)

概述

  • 产品名称
    Anti-PINK1抗体[38CT18.7]
    参阅全部 PINK1 一抗
  • 描述
    小鼠单克隆抗体[38CT18.7] to PINK1
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein corresponding to Human PINK1.

  • 阳性对照
    • WB: A431 cell lysate and mouse brain tissue lysate. IHC-P: Human kidney tissue. ICC/IF: PC-12 cells.

性能

应用

Our Abpromise guarantee covers the use of ab75487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 63 kDa.

靶标

  • 功能
    Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
  • 组织特异性
    Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
  • 疾病相关
    Defects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated.
  • 细胞定位
    Mitochondrion outer membrane. Cytoplasm > cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRPK antibody
    • FLJ27236 antibody
    • mitochondrial antibody
    • PARK 6 antibody
    • PARK6 antibody
    • Phosphatase and Tensin Homolog antibody
    • PINK 1 antibody
    • PINK1 antibody
    • PINK1_HUMAN antibody
    • Protein kinase BRPK antibody
    • PTEN induced putative kinase 1 antibody
    • PTEN induced putative kinase protein 1 antibody
    • PTEN-induced putative kinase protein 1 antibody
    • Serine/threonine kinase PINK1 mitochondrial antibody
    • Serine/threonine protein kinase PINK1 mitochondrial antibody
    • Serine/threonine-protein kinase PINK1 antibody
    see all

Anti-PINK1 antibody [38CT18.7] 图像

  • All lanes : Anti-PINK1 antibody [38CT18.7] (ab75487) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse IgG H&L at 1/5000 dilution

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human stomach section, labelling PINK1 with ab75487 (1/25). An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. 

  • Immunohistochemical analysis of paraffin-embedded Human heart section, labelling PINK1 with ab75487 (1/25). An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. 

  • Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling PINK1 (green) with ab75487 at a dilution of 1/25. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/400). Cytoplasmic actin was counterstained with Alexa Fluor® 555-conjugated with Phalloidin (red).

  • Immunocytochemistry/Immunofluorescence analysis of PC12 cells labelling PINK1 with ab75487. Cells were fixed with paraformaldehyde, permeabilization with 0.3X Triton X-100 and blocked with 10% serum for 1 hour at 4°C. Cells were incubated with the primary antibody at 2 µg/ml for 24 hours at 4°C. An Alexa Fluor® 488-conjugated anti-mouse (1/1000) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Anti-PINK1 antibody [38CT18.7] (ab75487)参考文献

This product has been referenced in:
  • Moretton A  et al. Selective mitochondrial DNA degradation following double-strand breaks. PLoS One 12:e0176795 (2017). WB . Read more (PubMed: 28453550) »
  • Cao S  et al. Melatonin-mediated mitophagy protects against early brain injury after subarachnoid hemorrhage through inhibition of NLRP3 inflammasome activation. Sci Rep 7:2417 (2017). WB . Read more (PubMed: 28546552) »

See all 7 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 14 2017

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification
liver, hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 06 2015

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse cardiomyocyte)
Specification
mouse cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% triton x100
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Dec 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC12 cell line)
Specification
PC12 cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1x PBS plus 0.3xTriton x
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Jun 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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