概述
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产品名称Anti-Pinin抗体
参阅全部 Pinin 一抗 -
描述兔多克隆抗体to Pinin
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宿主Rabbit
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经测试应用适用于: WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Cow, Dog
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Pinin.
(Peptide available as ab89383.)
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阳性对照
- This antibody gave a positive signal in Human Lung and Placenta tissue lysates.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab70605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 81 kDa). |
靶标
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功能Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.
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组织特异性Expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. Expressed strongly in melanoma metastasis lesions and advanced primary tumors.
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序列相似性Belongs to the pinin family.
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翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
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细胞定位Nucleus speckle. Cell junction > desmosome. Cell-cell contact area, predominantly desmosome of intercellular adherens junction. Not a nucleocytoplasmic shuttling protein.
- Information by UniProt
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数据库链接
- Entrez Gene: 403907 Dog
- Entrez Gene: 5411 Human
- Entrez Gene: 18949 Mouse
- Omim: 603154 Human
- SwissProt: P79149 Dog
- SwissProt: Q9H307 Human
- SwissProt: O35691 Mouse
- Unigene: 409965 Human
see all -
别名
- 140 kDa nuclear and cell adhesion-related phosphoprotein antibody
- Desmosome-associated protein antibody
- Domain-rich serine protein antibody
see all
图片
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Western blot - Anti-Pinin antibody (ab70605)All lanes : Anti-Pinin antibody (ab70605) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 87 kDa (why is the actual band size different from the predicted?)
Exposure time: 8 minutes
Pinin contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight (87 kDa) than predicted (81 kDa).
文献
ab70605 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"