RabMAb

Anti-Pin1抗体[EP1479Y] (ab76309)

概述

  • 产品名称Anti-Pin1抗体[EP1479Y]
    参阅全部 Pin1 一抗
  • 描述
    兔单克隆抗体[EP1479Y] to Pin1
  • 经测试应用适用于: WB, ICC, Flow Cytmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N terminal of human Pin1.

  • 阳性对照
    • 293 cell lysate.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab76309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ICC 1/100 - 1/250.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
    • 序列相似性Contains 1 PpiC domain.
      Contains 1 WW domain.
    • 结构域The WW domain is required for the interaction with STIL and KIF20B.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DOD antibody
      • DODO, Drosophila, homolog of antibody
      • FLJ40239 antibody
      • FLJ77628 antibody
      • MGC10717 antibody
      • NIMA interacting 1 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
      • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
      • Peptidyl-prolyl cis-trans isomerase pin1 antibody
      • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
      • Pin 1 antibody
      • Pin1 antibody
      • PIN1_HUMAN antibody
      • PPIase Pin1 antibody
      • Prolyl isomerase antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
      • Protein NIMA interacting 1 antibody
      • Rotamase Pin1 antibody
      • UBL 5 antibody
      • UBL5 antibody
      see all

    Anti-Pin1 antibody [EP1479Y] 图像

    • Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/500 dilution + 293 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
    • Overlay histogram showing HeLa cells stained with ab76309 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76309, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Pin1 antibody [EP1479Y] (ab76309)参考文献

    This product has been referenced in:
    • Guito J  et al. The cellular peptidyl-prolyl cis/trans isomerase Pin1 regulates reactivation of Kaposi's sarcoma-associated herpesvirus from latency. J Virol 88:547-58 (2014). IP . Read more (PubMed: 24173213) »
    • Ma SL  et al. Prolyl isomerase Pin1 promotes amyloid precursor protein (APP) turnover by inhibiting glycogen synthase kinase-3ß (GSK3ß) activity: novel mechanism for Pin1 to protect against Alzheimer disease. J Biol Chem 287:6969-73 (2012). IP ; Human . Read more (PubMed: 22184106) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Rat Ovary sections)
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: 0.05% Protease treatment for 5 mins @ 37' C
    Permeabilization No
    Specification Rat Ovary sections
    Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
    Fixative Formaldehyde
    Username

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    Verified customer

    提交于 Dec 04 2015

    The Pin1 antibody ab76309 has been tested for IF staining of cell cultures. We found that a dilution range of 1/100-1/250 produced the best result, with minimal background, using a fluorophore-conjugated secondary antibody for detection. But you may wa...

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    The price for product is same for Poland and Portugal and is given below;
    Product price = Euros 370
    Delivery price = Euros 42
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"