Anti-PIMT抗体(ab70559)
Key features and details
- Rabbit polyclonal to PIMT
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PIMT抗体 -
描述
兔多克隆抗体to PIMT -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla -
免疫原
Synthetic peptide corresponding a region between residue 150 and 200 of human PIMT (NP_079107.5).
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阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70559于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation. -
组织特异性
Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta. -
序列相似性
Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family. -
细胞定位
Cytoplasm. Nucleus > Cajal body. A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network. - Information by UniProt
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数据库链接
- Entrez Gene: 96764 Human
- Omim: 606461 Human
- SwissProt: Q96RS0 Human
- Unigene: 335068 Human
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别名
- Cap specific guanine N2 methyltransferase antibody
- Cap-specific guanine-N2 methyltransferase antibody
- CLL associated antigen KW 2 antibody
see all
图片
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PIMT was immunoprecipitated from HEK293T whole cell lysate (0.1 mg per IP reaction, 20% loaded) with ab70559 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70559 at 1 µg/mL.
Lane 1: ab70497 IP in HEK293T whole cell lysate.
Lane 2: ab70497 IP in HEK293T whole cell lysate.
Lane 3: rabbit anti-PIMT antibody IP in HEK293T whole cell lysate.
Lane 4: Control IgG in HEK293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PIMT with ab70559 at 1/200 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab70559 尚未被引用在任何文献中。