Anti-PIM3抗体- Carboxyterminal end (ab71321)

概述

  • 产品名称Anti-PIM3抗体- Carboxyterminal end
    参阅全部 PIM3 一抗
  • 描述
    兔多克隆抗体to PIM3 - Carboxyterminal end
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human PIM3 aa 259-288 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 415116

  • 阳性对照
    • WB: Mouse kidney tissue lysate IHC-P: Human hepatocarcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab71321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/10 - 1/50.
ELISA 1/1000.

靶标

  • 功能Able to phosphorylate CDKN1B in vitro. Involved in cell cycle progression and suppression of apoptosis. Implicated in proliferation of human hepatoma cell lines.
  • 组织特异性Widely expressed. No expression in colon, thymus, and small intestine. Expressed in human hepatoma cell lines but not in normal liver tissues. Expressed in colon cancer samples, particularly at the less advanced stages of cancer development.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Kid 1 antibody
    • Kid1 antibody
    • Kinase induced by depolarization antibody
    • ONCOGENE PIM3 antibody
    • PIM 3 antibody
    • Pim 3 oncogene antibody
    • Pim-3 proto-oncogene, serine/threonine kinase antibody
    • PIM3 antibody
    • Pim3 oncogene antibody
    • PIM3_HUMAN antibody
    • Protein kinase Kid 1 antibody
    • Protein kinase Kid1 antibody
    • Serine/threonine kinase Pim 3 antibody
    • Serine/threonine kinase Pim3 antibody
    • Serine/threonine protein kinase Pim 3 antibody
    • Serine/threonine protein kinase Pim3 antibody
    • Serine/threonine-protein kinase Pim-3 antibody
    see all

Anti-PIM3 antibody - Carboxyterminal end 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue sections labeling PIM3 - Carboxyterminal end with ab71321 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-PIM3 antibody - Carboxyterminal end (ab71321) at 1/1000 dilution + mouse kidney tissue lysate at 12.5 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 36 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PIM3 antibody - Carboxyterminal end (ab71321)参考文献

This product has been referenced in:
  • Selmi T  et al. ZFP36 expression impairs glioblastoma cell lines viability and invasiveness by targeting multiple signal transduction pathways. Cell Cycle 11:1977-87 (2012). WB ; Human . Read more (PubMed: 22544323) »

See 1 Publication for this product

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