概述

  • 产品名称Anti-PIM3抗体
    参阅全部 PIM3 一抗
  • 描述
    兔多克隆抗体to PIM3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human PIM3.

  • 阳性对照
    • This antibody gave a positive signal in Human Heart tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab108920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).

靶标

  • 功能Able to phosphorylate CDKN1B in vitro. Involved in cell cycle progression and suppression of apoptosis. Implicated in proliferation of human hepatoma cell lines.
  • 组织特异性Widely expressed. No expression in colon, thymus, and small intestine. Expressed in human hepatoma cell lines but not in normal liver tissues. Expressed in colon cancer samples, particularly at the less advanced stages of cancer development.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Kid 1 antibody
    • Kid1 antibody
    • Kinase induced by depolarization antibody
    • ONCOGENE PIM3 antibody
    • PIM 3 antibody
    • Pim 3 oncogene antibody
    • Pim-3 proto-oncogene, serine/threonine kinase antibody
    • PIM3 antibody
    • Pim3 oncogene antibody
    • PIM3_HUMAN antibody
    • Protein kinase Kid 1 antibody
    • Protein kinase Kid1 antibody
    • Serine/threonine kinase Pim 3 antibody
    • Serine/threonine kinase Pim3 antibody
    • Serine/threonine protein kinase Pim 3 antibody
    • Serine/threonine protein kinase Pim3 antibody
    • Serine/threonine-protein kinase Pim-3 antibody
    see all

Anti-PIM3 antibody 图像

  • All lanes : Anti-PIM3 antibody (ab108920) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human heart tissue lysate - total protein (ab29431) with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-PIM3 antibody (ab108920)参考文献

ab108920 has not yet been referenced specifically in any publications.

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