使用敲除细胞株进行验证

Anti-PIM1抗体[ZP003] (ab54503)

概述

  • 产品名称Anti-PIM1抗体[ZP003]
    参阅全部 PIM1 一抗
  • 描述
    小鼠单克隆抗体[ZP003] to PIM1
  • 特异性ab54503 is specific for the PIM1 protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from the internal region of the human PIM1 protein, which is 94% and 88% homologous with rat and mouse, respectively.

  • 阳性对照
    • WB: K562 cytoplasmic or nuclear lysate.

性能

应用

Our Abpromise guarantee covers the use of ab54503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa.
ELISA Use a concentration of 0.1 - 1 µg/ml.

靶标

  • 功能May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
  • 组织特异性Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated on both serine/threonine and tyrosine residues.
  • 细胞定位Cytoplasm. Nucleus and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Oncogene PIM 1 antibody
    • Oncogene PIM1 antibody
    • PIM 1 antibody
    • pim 1 kinase 44 kDa isoform antibody
    • Pim 1 kinase antibody
    • pim 1 oncogene (proviral integration site 1) antibody
    • Pim 1 oncogene antibody
    • PIM antibody
    • PIM1 antibody
    • pim1 kinase 44 kDa isoform antibody
    • PIM1_HUMAN antibody
    • Pim2 antibody
    • PIM3 antibody
    • Proto oncogene serine/threonine protein kinase Pim 1 antibody
    • Proto-oncogene serine/threonine-protein kinase Pim-1 antibody
    • Proviral integration site 1 antibody
    • Proviral integration site 2 antibody
    see all

Anti-PIM1 antibody [ZP003] 图像

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PIM1 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab54503 observed at 80 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab54503 was shown to recognize PIM1 when PIM1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PIM1 knockout samples were subjected to SDS-PAGE. ab54503 and ab181602 (loading control to GAPDH) were  diluted 3 μg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Anti-PIM1 antibody [ZP003] (ab54503) at 3 µg/ml + K562 cell lysate

    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-PIM1 antibody [ZP003] (ab54503)参考文献

ab54503 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Here are my answers to your questions. Is there a loading limit (cell number) for sample buffer as the lysis buffer? We recommend 1 ml of lysis buffer per 10 million cells. For sample buffer, if you mean gel loading b...

Read More

Thank you for contacting us with your question. It will not make a difference if the host of your secondary antibodies is the same or different, however if you will be using two primaries from different species on the same tissue, it will be bes...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"