概述

  • 产品名称
  • 描述
    兔多克隆抗体to PIGY
  • 特异性
    Only detects isoform 1 of PIGY.
  • 经测试应用
    适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    16 amino acid synthetic peptide from near the center of Human PIGY

  • 阳性对照
    • WB: Human liver and spleen tissue lysate. IF: Human spleen tissue. IHC-P: Human spleen tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 8 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性
    This gene encodes two proteins, one of which is part of the GPI-N-acetylglucosaminyltransferase (GIP-GnT) complex which initiates the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is synthesized in the endoplasmic reticulum and serves as an anchor for many surface proteins. Proteins containing GPI anchors can have an important role in cell-cell interactions. Two open reading frames have been found in the single transcript that has been identified for this gene. The downstream open reading frame encodes the GPI-GnT complex protein while the upstream open reading frame encodes a protein with unknown function.
  • 细胞定位
    Endoplasmic reticulum membrane. Plasma membrane.
  • 数据库链接
  • 别名
    • Phosphatidylinositol glycan anchor biosynthesis, class Y antibody
    • Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y antibody
    • Phosphatidylinositol-glycan biosynthesis class Y protein antibody
    • PIG Y antibody
    • PIG-Y antibody
    see all

图片

  • Immunofluorescence of PIGY in Human spleen tissue with ab79453 at 20 ug/mL.

  • Immunohistochemistry of Human spleen tissue using ab79453 at 2.5 μg/mL.

  • Lane 1 : Anti-PIGY antibody (ab79453) at 1 µg/ml
    Lane 2 : Anti-PIGY antibody (ab79453) at 2 µg/ml

    Lane 1 : human spleen tissue lysate
    Lane 2 : human spleen tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 8 kDa
    Despite its predicted molecular weight, PIGY often migrates at 28-30 kDa in SDS-PAGE.
  • Lane 1 : Anti-PIGY antibody (ab79453) at 1 µg/ml
    Lane 2 : Anti-PIGY antibody (ab79453) at 2 µg/ml

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human liver tissue lysate


    Predicted band size : 8 kDa

文献

ab79453 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab79453.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册