概述

  • 产品名称Anti-PIGY抗体
    参阅全部 PIGY 一抗
  • 描述
    兔多克隆抗体to PIGY
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    amino acids 23-114 (VARRCLHASG SRPLADRGKK TEEPPRDFDP ALLEFLVCPL SKKPLRYEAS TNELINEELG IAYPIIDGIP NMIPQAARMT RQSKKQEEVE QR) of Human PIGY.

  • 阳性对照
    • Human adrenal gland tissue; RT-4, U-251 MG, Human liver and tonsil lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

  • 相关性This gene encodes two proteins, one of which is part of the GPI-N-acetylglucosaminyltransferase (GIP-GnT) complex which initiates the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is synthesized in the endoplasmic reticulum and serves as an anchor for many surface proteins. Proteins containing GPI anchors can have an important role in cell-cell interactions. Two open reading frames have been found in the single transcript that has been identified for this gene. The downstream open reading frame encodes the GPI-GnT complex protein while the upstream open reading frame encodes a protein with unknown function.
  • 细胞定位Endoplasmic reticulum membrane. Plasma membrane.
  • 数据库链接
  • 别名
    • Phosphatidylinositol glycan anchor biosynthesis, class Y antibody
    • Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y antibody
    • Phosphatidylinositol-glycan biosynthesis class Y protein antibody
    • PIG Y antibody
    • PIG-Y antibody
    see all

Anti-PIGY antibody 图像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122338 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122338, at a 1/75 dilution, staining PIGY in paraffin-embedded Human adrenal gland tissue by immunohistochemistry.
  • All lanes : Anti-PIGY antibody (ab122338) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

Anti-PIGY antibody (ab122338)参考文献

ab122338 has not yet been referenced specifically in any publications.

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