概述

  • 产品名称Anti-PIGT抗体
    参阅全部 PIGT 一抗
  • 描述
    兔多克隆抗体to PIGT
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 432-481 (IQLPANSVTK VSIQFERALL KWTEYTPDPN HGFYVSPSVL SALVPSMVAA) of Human PIGT (NP_057021).

  • 阳性对照
    • Transfected 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

靶标

  • 相关性PIGT is an essential component of the multisubunit enzyme, GPI transamidase and is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells which serves to anchor proteins to the cell surface. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
  • 细胞定位Endoplasmic reticulum membrane; Single-pass type I membrane protein.
  • 数据库链接
  • 别名
    • CGI 06 antibody
    • FLJ41596 antibody
    • GPI transamidase component PIG T antibody
    • GPI transamidase subunit antibody
    • MGC8909 antibody
    • NDAP antibody
    • Neurotrophin regulated neuronal development associated protein antibody
    • Phosphatidyl inositol glycan class T antibody
    • Phosphatidylinositol glycan anchor biosynthesis class T antibody
    • Phosphatidylinositol glycan class T antibody
    see all

Anti-PIGT antibody 图像

  • Anti-PIGT antibody (ab80888) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)

Anti-PIGT antibody (ab80888)参考文献

ab80888 has not yet been referenced specifically in any publications.

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