Anti-PIGR抗体(ab96196)

Abreviews (4)Q&A (1)
Customer reviews and FAQs

概述

  • 产品名称Anti-PIGR抗体
    参阅全部 PIGR 一抗
  • 描述
    兔多克隆抗体to PIGR
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 180-429 of Human PIGR.

  • 阳性对照
    • 293T, A431, H1299, HeLa, HepG2, Molt4 and Raji cells; Cal27 xenograft tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/300. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
  • 序列相似性Contains 5 Ig-like V-type (immunoglobulin-like) domains.
  • 翻译后修饰N-glycosylation is not necessary for Ig binding.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Hepatocellular carcinoma associated protein TB6 antibody
      • Hepatocellular carcinoma-associated protein TB6 antibody
      • MGC125361 antibody
      • MGC125362 antibody
      • Phosphatidylinositol glycan, class R antibody
      • PIGR antibody
      • PIGR_HUMAN antibody
      • Poly Ig receptor antibody
      • Poly-Ig receptor antibody
      • Polymeric immunoglobulin receptor antibody
      • Secretory component antibody
      • Transmembrane secretory component antibody
      see all

    Anti-PIGR antibody 图像

    • Western blot - PIGR antibody (ab96196)
      Western blot - PIGR antibody (ab96196)
      Anti-PIGR antibody (ab96196) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg

      Predicted band size : 83 kDa
    • Immunocytochemistry/ Immunofluorescence - PIGR antibody (ab96196)
      Immunocytochemistry/ Immunofluorescence - PIGR antibody (ab96196)
      ab96196 at 1/200 dilution staining PIGR in methanol fixed HepG2 cells. Lower image is co-stained with Hoechst 33342.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIGR antibody (ab96196)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIGR antibody (ab96196)
      ab96196 at 1/500 dilution staining PIGR in paraffin-embedded Cal27 xenograft tissue.

    Anti-PIGR antibody (ab96196)参考文献

    ab96196 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab96196？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    提交 ab96196 的 abreview 提交 ab96196 的问题

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-PIGR antibody

     Excellent
    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Rat Hepatocytes)
    Permeabilization Yes - 0.2% Triton X-100 in PBS
    Specification Rat Hepatocytes
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Formaldehyde
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    Dr. Armen Petrosyan

    Verified customer

    提交于 Dec 21 2016

    Western blot abreview for Anti-PIGR antibody

     Good
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (Rat hepatocytes)
    Gel Running Conditions Reduced Denaturing (6% SDS-PAGE)
    Loading amount 15 µg
    Specification Rat hepatocytes
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Abcam user community

    Verified customer

    提交于 Nov 17 2016

    Western blot abreview for Anti-PIGR antibody

     Good
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (WIF-B cells, the hepatoma-derived hybrid cell line)
    Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
    Loading amount 35 µg
    Specification WIF-B cells, the hepatoma-derived hybrid cell line
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Username

    Abcam user community

    Verified customer

    提交于 Nov 04 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PIGR antibody

     Excellent
    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (WIF-B cells, the hepatoma-derived hybrid cell line)
    Permeabilization Yes - 0.2% Triton X-100
    Specification WIF-B cells, the hepatoma-derived hybrid cell line
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Formaldehyde
    Read More
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    Username

    Dr. Armen Petrosyan

    Verified customer

    提交于 Oct 17 2016

    Would like to try these antibodies with porcine samples.

    DISCOUNT CODES:

    For Ab17377: ***
    For Ab96196: ***

    Expiration date: February 18, 2013

    Value: $337 each

    Each of these codes will give you $337 off your next order before the expiration date. To redeem this off...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"