ab84624 detects isoform 1, 2, and 3.
Rat, Rabbit, Horse, Cow, Cat, Dog
Synthetic peptide corresponding to a region within N terminal amino acids 144-193 (PTVLPDRQAG ATTASTGGLA AVFDTVARSE VLFRSDRFDE GPVRLSHWQS) of human PIGQ (NP_004195).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.
Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Belongs to the PIGQ family.
Information by UniProt
- Phosphatidylinositol glycan, class Q antibody
- c407A10.1 antibody
- GPI1 antibody
Western blot - PIGQ antibody (ab84624)
Anti-PIGQ antibody (ab84624) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
65 kDaObserved band size:
60 kDa (why is the actual band size different from the predicted?
)Additional bands at:
55 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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