概述

  • 产品名称
  • 描述
    兔多克隆抗体to PIGK
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (10-30 amino acids in length) conjugated to KLH, from the N terminal of the first 100 amino acids of Human PIGK.

  • 阳性对照
    • 293 cell lysate, Human breast carcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab38445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 相关性
    PIGK is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
  • 细胞定位
    Endoplasmic reticulum, Endoplasmic reticulum membrane; type I single pass membrane protein
  • 数据库链接
  • 别名
    • GPI anchor transamidase antibody
    • GPI transamidase antibody
    • GPI8 antibody
    • Phosphatidylinositol glycan biosynthesis class K protein antibody
    • Phosphatidylinositol glycan class K antibody
    • PIG K antibody
    see all

图片

  • Anti-PIGK antibody (ab38445) at 1/100 dilution + 293 cell lysate

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • ab38445, at 1/50 dilution, staining PIGK in Human breast carcinoma by Immunohistochemistry, Formalin fixed and Paraffin embedded tissue. ab38445 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

文献

ab38445 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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