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Our Abpromise guarantee covers the use of ab110624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human PiGF.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to PIGF (Biotin) (ab84226). Using ab84226 as the detection antibody and ab110624 as the capture antibody, the detection limit for recombinant Human PiGF is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 25 kDa. The detection limit for recombinant Human PiGF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"