概述

  • 产品名称
  • 描述
    兔多克隆抗体to PiGF
  • 经测试应用
    适用于: Indirect ELISA, Sandwich ELISA, WBmore details
  • 种属反应性
    与反应: Recombinant fragment
    预测可用于: Human
  • 免疫原

    Highly pure (>98%) recombinant Human PiGF

性能

  • 形式
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab110624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human PiGF.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to PIGF (Biotin) (ab84226). Using ab84226 as the detection antibody and ab110624 as the capture antibody, the detection limit for recombinant Human PiGF is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 25 kDa. The detection limit for recombinant Human PiGF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

靶标

  • 功能
    Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.
  • 通路
    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • 序列相似性
    Belongs to the PIGF family.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GPI11 homolog antibody
    • MGC32646 antibody
    • MGC33136 antibody
    • PHOSPHATIDYLINOSITOL GLYCAN antibody
    • Phosphatidylinositol glycan biosynthesis class F protein antibody
    • Phosphatidylinositol-glycan biosynthesis class F protein antibody
    • PIG F antibody
    • PIG-F antibody
    • PIGF antibody
    • PIGF_HUMAN antibody
    • PIGF1 antibody
    see all

实验方案

Anti-PiGF antibody (ab110624)参考文献

This product has been referenced in:
  • Mohammed KA  et al. Cyclic stretch induces PlGF expression in bronchial airway epithelial cells via nitric oxide release. Am J Physiol Lung Cell Mol Physiol 292:L559-66 (2007). Read more (PubMed: 17028267) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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