Recombinant protein fragment corresponding to a region within amino acids 1 and 302 of PIG3 (NP_004872).
WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate;
ICC/IF: A549 cells.
Post translation modification: Acetylation
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
1/100 - 1/200.
May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. Has low NADPH-dependent diamine reductase activity (in vitro).
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
Information by UniProt
p53 induced gene 3 protein antibody
p53-induced gene 3 protein antibody
Putative quinone oxidoreductase antibody
Western blot - Anti-PIG3 antibody (ab96819)
Anti-PIG3 antibody (ab96819) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 35 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-PIG3 antibody (ab96819)
Immunofluorescence analysis of paraformaldehyde fixed A549 cells, using ab96819 at 1/200 dilution: (1) without DNA probe; (2) merged with DNA probe.
has not yet been referenced specifically in any publications.
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