猪IL-6 (Interleukin-6) ELISA试剂盒(ab100755)

概述

  • 产品名称猪IL-6 (Interleukin-6) ELISA试剂盒
    参阅全部 IL6 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 45 pg/ml
  • 范围
    40.96 pg/ml - 10000 pg/ml
  • 复检

    89 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 108.3 95% - 117%
    Serum 93.47 85% - 102%
    Plasma 85.73 67% - 99%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Pig
  • 产品概述

    Abcam's IL-6 (Interleukin-6) Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL6 in serum, plasma and cell culture supernatants. 

    This assay employs an antibody specific for pig IL6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 疾病相关Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 序列相似性Belongs to the IL-6 superfamily.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating fact
    • HGF
    • HPGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6 protein
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    • Interleukin6
    see all

应用

Our Abpromise guarantee covers the use of ab100755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Pig IL-6 (Interleukin-6) ELISA Kit 图像

  • Representative Standard Curve using ab100755

实验方案

Pig IL-6 (Interleukin-6) ELISA Kit (ab100755)参考文献

This product has been referenced in:
  • Choi CY  et al. The ORF3 protein of porcine circovirus type 2 promotes secretion of IL-6 and IL-8 in porcine epithelial cells by facilitating proteasomal degradation of regulator of G protein signalling 16 through physical interaction. J Gen Virol 96:1098-108 (2015). Read more (PubMed: 25575706) »
  • Kwit K  et al. The influence of experimental infection of gilts with swine H1N2 influenza A virus during the second month of gestation on the course of pregnancy, reproduction parameters and clinical status. BMC Vet Res 10:123 (2014). ELISA ; Pig . Read more (PubMed: 24893655) »

See all 3 Publications for this product

Product Wall

As an example, Il-6 in the tested pig plasma is ˜2ng/ml.

I can confirm that the requested kits (ab100755, ab100756 and ab113352) are compatible with citrate as anticoagulant. The sensitivity will not change due to different anticoagulants.

Abreview for IL6 Pig ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Pig Tissue sections (Brain)
Specification: Brain
Amount of Sample: 500 µg
Type of Kit Used: ELISA
Positive control: WE DO NOT HAVE A POSITIVE CONTROL
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

提交于 Oct 16 2012

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I am very pleased to hear you would like to accept our offer and test ab100755 in Pig tissue samples. This code will give you 1 free kit or value off of order before the expiration date. To...

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Gracias por contactarnos.

Me comentan mis compañeros que aunque este kit nunca se ha testado con tejidos, dada su versatilidad con distintos tipos de muestras, es muy posible que también funcione con homogenado de cerebro.
...

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I have received information regarding

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ab119590 Cystatin C Mouse ELISA Kit
ab119596 KIM1 Mouse ELISA Kit
ab119597 KIM1 Rat ELISA Kit
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Thank you for contacting us.


To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"