猪IL-1 beta ELISA试剂盒(ab100754)

概述

  • 产品名称猪IL-1 beta ELISA试剂盒
    参阅全部 IL1 beta 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 6 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 96.91 89% - 104%
    Serum 97.32 88% - 105%
    Plasma 87.75 80% - 92%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Pig
  • 产品概述

    Abcam's IL1 beta Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of pig IL1 beta in serum, plasma and cell culture supernatants.


     


    This assay employs an antibody specific for IL1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    For higher sensitivity and reproducibility in only 90 minutes, we recommend Mouse IL-1 beta ELISA Kit (ab197742) from our SimpleStep ELISA® range.


     

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 序列相似性Belongs to the IL-1 family.
  • 结构域The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 细胞定位Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 别名
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all

相关产品

应用

Our Abpromise guarantee covers the use of ab100754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Pig IL-1 beta ELISA Kit 图像

  • Representative Standard Curve using ab100754

实验方案

Pig IL-1 beta ELISA Kit (ab100754)参考文献

ab100754 has not yet been referenced specifically in any publications.

Product Wall

Application ELISA
Sample Pig Serum (cell extracts)
Type of Kit Used ELISA
Specification cell extracts
Did you use the Abcam Kit protocol? Yes
Amount of Sample 100 µg
Positive control standard provided by kit
Username

Dr. Carolina Velez

Verified customer

提交于 Feb 22 2016

Abreview for IL1 beta Pig ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Pig Serum (Blood)
Specification: Blood
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Standards IL1 Beta
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Serum samples were analyzed undiluted, all samples were within the detection range of the kit, and the calibration curve was well adjusted (R2: 0.9992).
Username

Abcam user community

Verified customer

提交于 Mar 26 2013

Thank you for contacting us.

I have received following reply from my colleague in laboratory;

I’m not exactly sure what the customer means by “one recovery at a high concentration” as the Spiking and Recovery QC data...

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Thank you for contacting us.

This kit is developed by one of our collaborator. Our collaborator product as this one has passed strict quality checks we have at Abcam which is why we are selling this kit. The product has been fully tested with ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"