抗猪Albumin抗体(ab79960)

Abreviews (2)Q&A (3)

概述

  • 产品名称
    抗猪Albumin抗体
  • 描述
    兔多克隆抗体to猪Albumin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, RIA, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Pig
  • 免疫原

    Pig Albumin purified from pig plasma

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.5
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
RIA Use a concentration of 5 µg/ml.
ELISA Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性
    Albumin is a soluble, monomeric protein that makes up about half of the blood serum protein. It is a carrier protein for steroids, fatty acids, and thyroid hormones and also plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
  • 细胞定位
    Secreted
  • 数据库链接
    • 别名
      • ALB antibody
      • Albumin antibody
      • Serum albumin antibody

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pig Albumin antibody (ab79960)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pig Albumin antibody (ab79960)This image is courtesy of an Abreview submitted by Oscar McCook

      ab79960 staining Pig Albumin in pig kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/500) for 1 hour at 22°C. An undiluted Alkaline Phosphatase-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    文献

    ab79960 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab79960？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    5 reviews or Q&A

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Pig Albumin antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated
    Sample
    Pig Tissue sections (kidney and heart)
    Specification
    kidney and heart
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
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    Username

    Oscar Mccook

    Verified customer

    提交于 Sep 23 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pig Albumin antibody

     Average
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (porine periodontal ligament cells)
    Specification
    porine periodontal ligament cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 1% PBST
    Blocking step
    1% serum + 0.1% Tween-20 +PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 99.9% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 Feb 28 2012

    Does this antibody cross react with human?

    Thank you for contacting Abcam.

    I have heard back from the lab and they have told me that this antibody has been tested against Human, Mouse, Rat, Monkey, and Canine plasma. As well as Rabbit and Bovine serum. There was a less than 0.01% cros...

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    Dear John I am very much interested in this. How should I do this?? Do I go online to fill out Abreview on this product?? Thank you so much for all your help.

    Thank you for your reply. As I mentioned before normally the discount code has to be issued before purchasing the antibody, in this case I have made an exception. To redeem the discount code below, you must first submit an Abreview on our website (plea...

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    To whom it may concern: Hello, I purchased Rabbit polyclonal to pig Albumin antibody (ab79960) to immunostain porcine periodontal ligament epithelial cells which were co-cultured with mouse fibroblast cells to distinguish one cell from the other. Howev...

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    Thank you for contacting Abcam. The anti-Pig Albumin antibody has not previously been tested in ICC/IF and so we have no standard protocol for this particular antibody, but I have looked over the protocol you sent and have a few questions/tips that may...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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